ARPC2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA944847A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: JURKAT whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), HepG2 whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), Mouse brain tissue lysate(20µg), Rat brain tissue lysate(20µg) All lanes: ARPC2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time：20s Immunofluorescence staining of Hela cell with CSB-RA944847A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA944847A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

O15144

Target Names

ARPC2

Alternative Names

Actin related protein 2/3 complex subunit 2 34kDa antibody; Actin related protein 2/3 complex subunit 2 antibody; Actin-related protein 2/3 complex subunit 2 antibody; ARC 34 antibody; ARC34 antibody; Arp2/3 complex 34 kDa subunit antibody; ARP2/3 protein compex subunit 34 antibody; ARPC 2 antibody; Arpc2 antibody; ARPC2_HUMAN antibody; p34 Arc antibody; p34-ARC antibody; p34Arc antibody; PNAS 139 antibody; PNAS139 antibody; PRO 2446 antibody; PRO2446 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human ARPC2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12C10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ARPC2, also known as p34-Arc, is a critical component of the Arp2/3 complex that drives actin filament nucleation and branching at the leading edge of motile cells. This 34 kDa subunit plays essential roles in cell migration, endocytosis, and intracellular trafficking, making it a key target for researchers investigating cytoskeletal dynamics, cancer metastasis, and cellular morphogenesis.

This recombinant monoclonal antibody, generated from clone 12C10 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than traditional hybridoma methods, researchers benefit from lot-to-lot uniformity that supports reliable data across long-term studies and multi-site collaborations.

Validation testing demonstrates robust performance across multiple applications and sample types. Western blot analysis detects a clean 34 kDa band matching the predicted molecular weight across a diverse panel of human cell lines including Jurkat, COLO-205, HepG2, HeLa, K562, and HEK293, with effective detection at 1:1000 dilution. Cross-species reactivity extends to rodent samples, with confirmed detection in both mouse and rat brain tissue lysates, supporting translational research using animal models. Immunofluorescence staining in HeLa cells reveals characteristic cytoplasmic localization patterns consistent with ARPC2's role in actin network organization. Flow cytometry validation further demonstrates utility for quantitative single-cell analysis of ARPC2 expression levels.

This antibody serves researchers studying actin cytoskeleton regulation, cell motility mechanisms, and related processes in cancer biology, neuroscience, and developmental biology, providing a reliable tool for interrogating Arp2/3 complex function across experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Actin-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the mother actin filament. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

Gene References into Functions

In the association analysis of 110 gastric cancer tissues, ARPC2 showed significant associations with large tumor size, lymph node invasion, and high tumor stage. In addition, ARPC2-positive patients exhibited lower RFS and OS rates compared with ARPC2-negative patients. We thus identify that ARPC2 plays an aneretic role in human gastric cancer and provided a new target for gastric cancer therapy. PMID: 28694563
Downregulation of the ARP2/3 complex signaling pathway, a common final pathway for multiple signaling cascades that regulate the actin cytoskeleton, would compromise the structural stability of spines, leading to their loss. In concert with findings from deletion of the ARP2/3 complex in mice, these findings support the idea that spine deficits in the DLPFC may contribute to subcortical hyperdopaminergia in schizophrenia. PMID: 27523502
Recent reports now demonstrate a novel aspect of the ARP2/3 complex and the nucleating-promoting factors in the maintenance of endothelial barrier function and junction remodeling of established endothelial cell junctions. PMID: 24621569
FOXF1 repressed cell growth and expression of collagen-1 and actin-related protein 2/3 complex, subunit 2. PMID: 25260753
The Arp2/3 complex is recruited to invading Rickettsia parkeri and is required for efficient invasion. PMID: 22188208
Filopodia initiation: focus on the Arp2/3 complex and formins PMID: 21975549
WASH is a bimodular protein and a component of the BLOC-1 complex in which the C terminus is involved in Arp2/3-mediated actin nucleation, whereas the N-terminal portion is required for its regulation and localization in the cells PMID: 20308062
The actin-related protein 2 accumulated in punctate structures that formed an extensivenetwork in a region corresponding to the postition of the Golgi complex. PMID: 15793564
Compared with controls, actin-related protein 2/3 level was markedly increased in brains of intractable epilepsy patients. PMID: 18708039
These findings strongly suggest that defective IL10 function is central to the pathogenesis of the ulcerative colitis subtype of inflammatory bowel disease. PMID: 18836448

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Subcellular Location

Cytoplasm, cytoskeleton. Cell projection. Cell junction, synapse, synaptosome. Nucleus.

Protein Families

ARPC2 family

Database Links

HGNC: 705

OMIM: 604224

KEGG: hsa:10109

STRING: 9606.ENSP00000295685

UniGene: Hs.529303

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Related pathways

Regulation of actin cytoskeleton Fc gamma R-mediated phagocytosis Endocytosis

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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