Acetyl-Histone H2A type 1-B/E (K9) Recombinant Monoclonal Antibody

Histone H2A acetylation at lysine 9 represents a critical epigenetic modification that influences chromatin accessibility and transcriptional regulation. This post-translational modification serves as a key marker for understanding how cells modulate gene expression programs, making it an essential target for researchers investigating chromatin dynamics, cell cycle regulation, and epigenetic mechanisms underlying disease states.

This recombinant monoclonal antibody, clone 3H5, offers researchers the reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures consistent performance across lots, eliminating the variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, combined with affinity chromatography purification, delivers a reagent optimized for specificity in detecting acetylated H2A type 1-B/E in human samples.

Validation studies demonstrate robust performance in both immunocytochemistry and immunofluorescence applications. In HeLa cells, the antibody produces clear nuclear staining patterns consistent with the expected chromatin localization of acetylated histones. Notably, immunofluorescence experiments using sodium butyrate-treated HeLa cells, which inhibits histone deacetylases and enriches acetylation marks, confirm the antibody's ability to detect enhanced acetylation signals under conditions that elevate this modification. This functional validation provides confidence that the antibody responds appropriately to biologically relevant changes in acetylation status.

The flexible dilution ranges accommodate optimization across different experimental setups, with immunocytochemistry applications working well between 1:50 and 1:500, while immunofluorescence protocols perform effectively at 1:30 to 1:200. For researchers exploring epigenetic regulation, nuclear signaling pathways, or chromatin biology, this antibody provides a dependable tool for interrogating H2A acetylation in human cell systems.