Acetyl-Histone H2A type 1-B/E (K9) Recombinant Monoclonal Antibody

Code CSB-RA010385A09acHU
Size US$210
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  • Immunocytochemistry analysis of CSB-RA010385A09acHU diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
  • Immunofluorescence staining of Hela cells(treated by 15mM sodium butyrate for 30min) with CSB-RA010385A09acHU at 1:56,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Product Details

Uniprot No.
Target Names
HIST1H2AB
Alternative Names
H2a 615 antibody; H2A antibody; H2A GL101 antibody; H2A histone family member A antibody; H2A.1 antibody; H2A.2 antibody; H2A/a antibody; H2A/m antibody; H2A/O antibody; H2A/q antibody; H2A1B_HUMAN antibody; H2AFA antibody; H2AFE antibody; H2AFL antibody; H2AFM antibody; H2AFO antibody; H2AFQ antibody; HIST1H2AE antibody; HIST1H2AJ antibody; HIST2H2AA antibody; HIST2H2AA3 antibody; HIST2H2AB antibody; HIST2H2AC antibody; Histone 1 H2ae antibody; Histone 2 H2aa3 antibody; Histone 2 H2ab antibody; Histone 2 H2ac antibody; Histone H2A type 1 B antibody; Histone H2A type 1 C antibody; Histone H2A type 1 E antibody; Histone H2A type 1 J antibody; Histone H2A type 1-B/E antibody; Histone H2A.2 antibody; Histone H2A/a antibody; Histone H2A/m antibody; MGC74460 antibody
Species Reactivity
Human
Immunogen
A synthesized peptide
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
3H5
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, ICC, IF
Recommended Dilution
Application Recommended Dilution
ICC 1:50-1:500
IF 1:30-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

Creating the acetyl-Histone H2A type 1-B/E (K9) recombinant monoclonal antibody involves cloning the genes encoding the HIST1H2AB antibody, including both heavy and light chains. These cloned genes are inserted into expression vectors, which are then introduced into host cells through transfection. The host cells take on the responsibility of producing and secreting the antibody. The antibody is subsequently purified through affinity chromatography to ensure its purity and effectiveness. Rigorous functionality testing in applications like ELISA, ICC, and IF confirms its suitability for the precise detection of the human HIST1H2AB protein acetylated at K9.

H2A type 1-B/E K9 acetylation is primarily associated with transcriptional activation. It helps recruit transcriptional activators, coactivators, and chromatin remodeling complexes to gene promoters. This enhances the initiation and progression of transcription, leading to increased gene expression. Acetylation of H2A type 1-B/E at K9 is involved in DNA repair, epigenetic signaling, and coordinated gene regulation.

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Target Background

Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Subcellular Location
Nucleus. Chromosome.
Protein Families
Histone H2A family
Database Links

HGNC: 4734

OMIM: 602786

KEGG: hsa:3012

STRING: 9606.ENSP00000303373

UniGene: Hs.121017

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