Histone H2A type 1-B/E Recombinant Monoclonal Antibody

Histone H2A type 1-B/E is a core component of the nucleosome, the fundamental repeating unit of chromatin that packages eukaryotic DNA. As one of the canonical H2A variants, it plays essential roles in chromatin structure, DNA repair, and transcriptional regulation. Understanding histone biology is central to epigenetics research, where modifications to these proteins serve as key regulatory signals that influence gene expression without altering the underlying DNA sequence.

This recombinant monoclonal antibody, clone 1H12, offers researchers the reproducibility and consistency that modern experimental workflows demand. Because recombinant antibodies are produced from defined genetic sequences rather than traditional hybridoma methods, you can expect reliable performance across experiments and between lots, eliminating a common source of variability in chromatin studies.

The antibody has been validated for multiple applications, giving you flexibility in your experimental approach. Western blot analysis in HepG2 whole cell lysate demonstrates clean detection at the expected 13 kDa molecular weight, confirming specificity for the target protein. For researchers investigating subcellular localization, immunofluorescence staining in HeLa cells reveals the expected nuclear distribution pattern characteristic of core histones, with clear signal when counter-stained with DAPI. The antibody is also suitable for ELISA-based detection methods.

Raised in rabbit and affinity-purified for enhanced specificity, this reagent is validated for human samples and arrives in a stabilized liquid format ready for immediate use. Whether you are mapping histone dynamics, studying chromatin remodeling complexes, or investigating epigenetic mechanisms in nuclear signaling pathways, this antibody provides a dependable tool for your research.