Hydroxyl-Histone H2A type 1-B/E (Y39) Recombinant Monoclonal Antibody

Histone H2A type 1-B/E serves as a fundamental component of the nucleosome, the basic structural unit of chromatin that packages DNA within the nucleus. The hydroxylation of tyrosine 39 on this histone variant represents a post-translational modification with emerging significance in epigenetic regulation, influencing chromatin accessibility and gene expression patterns. Studying this specific modification provides researchers with insights into the dynamic interplay between chromatin structure and cellular function.

This recombinant monoclonal antibody, clone 3B12, offers the consistency and reliability that demanding epigenetic research requires. Produced using recombinant technology in rabbit host, each lot delivers sequence-defined specificity against the hydroxylated Y39 epitope, eliminating the batch-to-batch variability that can complicate longitudinal studies or multi-site collaborations. The affinity-chromatography purification ensures high purity, supporting cleaner backgrounds in your experiments.

Validation in Western blot applications demonstrates robust performance, with the antibody detecting the expected 14 kDa band in mouse spleen tissue at working dilutions between 1:500 and 1:2000. This concordance between predicted and observed molecular weight confirms specific recognition of the target protein. The antibody is also validated for ELISA, providing flexibility for researchers who need to quantify hydroxyl-H2A levels across experimental conditions.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this unconjugated format allows pairing with your preferred secondary detection system. For investigators exploring histone modifications, chromatin dynamics, or broader questions in epigenetics and nuclear signaling, this antibody provides a dependable tool for interrogating hydroxyl-H2A biology with confidence.