Code | CSB-RA010418A04acHU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
ICC | 1:50-1:500 |
IF | 1:30-1:200 |
In the quest to produce the acetyl-histone H3.1 (K4) recombinant monoclonal antibody, the initial phase involves the extraction of genes encoding the HIST1H3A antibody from rabbits that have been previously exposed to a synthesized peptide derived from the human HIST1H3A protein acetylated at K4. These antibody genes are then seamlessly integrated into specialized expression vectors. Following this genetic modification, the modified vectors are introduced into host suspension cells, which are carefully cultured to stimulate the expression and secretion of antibodies. Subsequently, the HIST1H3A recombinant monoclonal antibody is subjected to a meticulous purification process utilizing affinity chromatography techniques, effectively isolating the antibody from the surrounding cell culture supernatant. Finally, the functionality of the antibody is comprehensively assessed through a diverse range of assays, including ELISA, WB, ICC, and IF tests, unequivocally confirming its ability to interact effectively with the human HIST1H3A protein acetylated at K4.
Acetylation of HIST1H3A at K4 is a key epigenetic modification that promotes an open chromatin structure and activates gene expression. It plays a central role in transcriptional regulation, cellular differentiation, and the maintenance of gene expression patterns across generations of cells. Dysregulation of this modification can have significant implications for health and disease.
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