BCL2L14 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA012968A0HU
Size	US$210
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Image	IHC image of CSB-RA012968A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HepG2 cell with CSB-RA012968A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing jurkat cells stained with CSB-RA012968A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

Q9BZR8

Target Names

BCL2L14

Alternative Names

BCL2L14 antibody; BCLGApoptosis facilitator Bcl-2-like protein 14 antibody; Bcl2-L-14 antibody; Apoptosis regulator Bcl-G antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human BCL2L14

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9H1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BCL2L14, also known as Bcl-G, belongs to the BCL-2 family of apoptosis regulators and functions as a pro-apoptotic protein that promotes programmed cell death. This apoptosis facilitator plays important roles in tissue homeostasis and has garnered research interest for its involvement in cancer biology, where dysregulation of apoptotic pathways contributes to tumor development and therapeutic resistance. Understanding BCL2L14 expression and localization provides valuable insights into cell death mechanisms across various tissue contexts.

This recombinant monoclonal antibody, clone 9H1, offers the reproducibility and consistency that demanding research applications require. Produced using recombinant technology with a defined sequence, this rabbit IgG eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. Researchers can confidently build upon previous results knowing that antibody performance remains stable across experiments and over time.

Validation across multiple detection platforms demonstrates this antibody's versatility in your experimental workflows. Immunohistochemistry studies in paraffin-embedded human testis tissue at dilutions of 1:50-1:200 reveal specific staining patterns suitable for tissue expression analysis. Immunofluorescence applications have been validated in HepG2 hepatocellular carcinoma cells, enabling subcellular localization studies with clear signal when counter-stained with DAPI. Flow cytometry validation using Jurkat T lymphocyte cells confirms utility for quantitative single-cell analysis, with overlay histograms demonstrating distinct positive population shifts compared to isotype controls.

This antibody serves researchers investigating apoptotic signaling pathways, cancer cell biology, and tissue-specific expression patterns of BCL-2 family members. The combination of recombinant production, multi-platform validation, and human reactivity makes this reagent well-suited for studies exploring BCL2L14's role in cell fate decisions and disease pathogenesis.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Plays a role in apoptosis.

Gene References into Functions

Data indicate that LRP6, BCL2L14, DUSP16, CREBL2, and CDKN1B were involed in centromeric (12p11.21-12p13.2) deletion in ETV6-RUNX1 B-cell precursor acute lymphoblastic leukemia (BCP-ALL). PMID: 23077088
Single nucleotide polymorphism in BCL2L14 is associated with lung cancer. PMID: 22573796
prior knockdown of Bcl-G expression ablates the stimulation of basal apoptosis by FAU, consistent with an essential downstream role for Bcl-G, itself a candidate tumour suppressor, in mediating the apoptosis regulatory role of FAU. PMID: 21550398
siRNA downregulation of Bcl-G inhibited breast cancer cell apoptosis. Adding an siRNA against Fau revealed control of Bcl-G by Fau. The most important factors controlling Bcl-G are post-translational modification by Fau & MELK, not transcription rate. PMID: 19671159
There was no somatic mutation of BH3 domains of Bad, Bmf and Bcl-G genes in transitional cell carcinoma samples. The data presented here indicate that BH3 domain mutation of these genes is rare in TCCs and may not contribute to the pathogenesis of TCCs. PMID: 16484005
the kinase activity of MELK is likely to affect mammary carcinogenesis through inhibition of the pro-apoptotic function of Bcl-GL PMID: 17280616
data presented here indicate that BH3 domain mutation of the proapoptotic genes Bad, Bmf and Bcl-G is rare in laryngeal squamous cell carcinoma and may not contribute to the apoptosis-resistance mechanisms of laryngeal squamous cell carcinoma PMID: 17557568
JAB1 is involved in the regulation of mitochondrial apoptotic pathway through specific interaction with BclGs. PMID: 18006276
Increased BclG(L) expression may contribute to the aberrant CD4+ T cell apoptosis which causes an inappropriate immune response and impaired homeostasis in systemic lupus erythematosus. PMID: 19524489

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Subcellular Location

Cytoplasm.; [Isoform 1]: Cytoplasm, cytosol. Note=Diffusely distributed throughout the cytosol.; [Isoform 2]: Endomembrane system. Note=Predominantly localized to cytosolic organelles.

Protein Families

Bcl-2 family

Tissue Specificity

Isoform 1 is widely expressed. Isoform 2 is testis-specific.

Database Links

HGNC: 16657

OMIM: 606126

KEGG: hsa:79370

STRING: 9606.ENSP00000309132

UniGene: Hs.210343

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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