BMI1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA919832A0HU
Size	US$210
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Image	Flow cytometric analysis of Bmi1 expression in HepG2 cells using Bmi1 antibody. Green, isotype control; red, Bmi1. Western blotting analysis using Bmi1 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Bmi1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P35226

Target Names

BMI1

Alternative Names

BMI1; BMI1 Proto-Oncogene, Polycomb Ring Finger; RNF51; PCGF4; Polycomb Group RING Finger Protein 4; Polycomb Complex Protein BMI-1; B Lymphoma Mo-MLV Insertion Region 1 Homolog (Mouse); Murine Leukemia Viral (Bmi-1) Oncogene Homolog; B Lymphoma Mo-MLV Insertion Region 1 Homolog; BMI1 Polycomb Ring Finger Proto-Oncogene; BMI1 Polycomb Ring Finger Oncogene; Polycomb Group Ring Finger 4; Polycomb Group Protein Bmi1; Ring Finger Protein 51; RING Finger Protein 51; Flvi-2/Bmi-1; FLVI2/BMI1

Species Reactivity

Human, Rat

Immunogen

Recombinant Human BMI1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15H3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
FC	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A. In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Subcellular Location

Nucleus. Cytoplasm.

Database Links

HGNC: 1066

OMIM: 164831

KEGG: hsa:100532731

STRING: 9606.ENSP00000365851

UniGene: Hs.380403

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Related pathways

Cell markers Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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