C19orf10 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA842625MA1HU
Size	US$210
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Image	IHC image of CSB-RA842625MA1HU diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Anti-Human lgG, Fcy Fragment Specific labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing PC-3 cells stained with CSB-RA842625MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q969H8

Target Names

C19orf10

Alternative Names

C19orf10 antibody; Chromosome 19 open reading frame 10 antibody; CS010_HUMAN antibody; EUROIMAGE1875335 antibody; IL 25 antibody; IL 27 antibody; IL-25 antibody; IL25 antibody; IL27 antibody; IL27w antibody; Interleukin 25 antibody; Interleukin 27 working designation antibody; Interleukin-25 antibody; Interleukin25 antibody; R33729 1 antibody; R337291 antibody; SF20 antibody; Stromal cell derived growth factor antibody; Stromal cell derived growth factor SF20 antibody; Stromal cell-derived growth factor SF20 antibody; Uncharacterized protein C19orf10 precursor antibody; UPF0556 protein C19orf10 antibody

Species Reactivity

Human

Immunogen

Recombinant Human C19orf10 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

15G9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Bone marrow-derived monocyte and paracrine-acting protein that promotes cardiac myocyte survival and adaptive angiogenesis for cardiac protection and/or repair after myocardial infarction (MI). Stimulates endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway. Inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway. Involved in endothelial cell proliferation and angiogenesis.

Gene References into Functions

Bone marrow-derived monocytes and macrophages produce this protein endogenously to protect and repair the heart after myocardial infarction. PMID: 25581518
Taken together, these data suggest that c19orf10 might be one of the growth factors and potential molecular targets activated in HCC. PMID: 21128247
we identified a novel protein, c19orf10 (chromosome 19 open reading frame 10), produced by fibroblast-like synoviocytes PMID: 17362502

Subcellular Location

Secreted. Endoplasmic reticulum-Golgi intermediate compartment. Endoplasmic reticulum. Golgi apparatus.

Protein Families

MYDGF family

Tissue Specificity

Expressed in eosinophils (at protein level). Expressed in bone marrow cells. Expressed in synovial tissue. Found in synovial fluid of patients with arthropaties.

Database Links

HGNC: 16948

OMIM: 606746

KEGG: hsa:56005

STRING: 9606.ENSP00000262947

UniGene: Hs.465645

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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