CALCA Recombinant Monoclonal Antibody

Calcitonin, encoded by the CALCA gene, serves as a critical peptide hormone involved in calcium homeostasis and bone metabolism. Beyond its classical endocrine functions, CALCA and its processed derivatives—including calcitonin and katacalcin—have garnered significant research interest in signal transduction pathways and as biomarkers in various pathological conditions, particularly in thyroid-related studies where calcitonin serves as a key diagnostic indicator.

This recombinant monoclonal antibody, clone 17H10, offers researchers the reproducibility and consistency that sequence-defined recombinant technology provides. Unlike traditional hybridoma-derived antibodies, recombinant production ensures lot-to-lot uniformity, allowing you to confidently compare results across extended studies without concerns about batch variation affecting your data interpretation.

The antibody has been validated for multiple applications, giving you flexibility across different experimental workflows. Immunohistochemistry studies have demonstrated clear detection in paraffin-embedded human thyroid tissue at dilutions of 1:50 to 1:200, with antigen retrieval performed using citrate buffer under high pressure conditions. Flow cytometry validation using A549 cells confirms intracellular detection capability, with the overlay histogram showing distinct positive signal separation from isotype control when cells were fixed and permeabilized prior to staining. The antibody has also been validated for ELISA applications.

Supplied in a liquid format containing glycerol and PBS with Proclin 300 as a preservative, this unconjugated antibody provides flexibility for detection system selection based on your experimental requirements. The human IgG1 isotype format, combined with affinity chromatography purification, ensures high purity suitable for demanding applications in signal transduction research and related investigations into CALCA biology.