Alternative Names
CALCA ELISA kit; CALC1Calcitonin [Cleaved into: Calcitonin; Katacalcin ELISA kit; Calcitonin carboxyl-terminal peptide ELISA kit; CCP ELISA kit; PDN-21)] ELISA kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.688 pg/mL-300 pg/mL
Detection Wavelength
450 nm
Research Area
Signal Transduction
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of human CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
81-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
92-100 |
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| 1:4 |
Average % |
94 |
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| Range % |
90-110 |
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| 1:8 |
Average % |
94 |
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| Range % |
89-105 |
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Recovery
| The recovery of human CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
85-100 |
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| EDTA plasma (n=4) |
92 |
88-99 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 300 |
2.379 |
2.252 |
2.316 |
2.173 |
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| 150 |
1.794 |
1.760 |
1.777 |
1.634 |
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| 75 |
1.305 |
1.284 |
1.295 |
1.152 |
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| 37.5 |
0.954 |
0.909 |
0.932 |
0.789 |
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| 18.7 |
0.593 |
0.564 |
0.579 |
0.436 |
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| 9.4 |
0.372 |
0.349 |
0.361 |
0.218 |
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| 4.7 |
0.253 |
0.239 |
0.246 |
0.103 |
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| 0 |
0.149 |
0.137 |
0.143 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
