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Human Calcitonin,CT ELISA Kit

Instructions
Code CSB-E05131h
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Calcitonin
Alternative Names CALCA ELISA kit; CALC1Calcitonin [Cleaved into: Calcitonin; Katacalcin ELISA kit; Calcitonin carboxyl-terminal peptide ELISA kit; CCP ELISA kit; PDN-21)] ELISA kit
Abbreviation CALCA
Uniprot No. P01258
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.688 pg/mL-300 pg/mL
Sensitivity 1.172 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 88  
Range % 81-95  
1:2 Average % 95  
Range % 92-100  
1:4 Average % 94  
Range % 90-110  
1:8 Average % 94  
Range % 89-105  
Recovery
The recovery of human CT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 85-100  
EDTA plasma (n=4) 92 88-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
300 2.379 2.252 2.316 2.173  
150 1.794 1.760 1.777 1.634  
75 1.305 1.284 1.295 1.152  
37.5 0.954 0.909 0.932 0.789  
18.7 0.593 0.564 0.579 0.436  
9.4 0.372 0.349 0.361 0.218  
4.7 0.253 0.239 0.246 0.103  
0 0.149 0.137 0.143 　  
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.; FUNCTION
Subcellular Location Secreted
Protein Families Calcitonin family
Database Links

HGNC: 1437

OMIM: 114130

KEGG: hsa:796

STRING: 9606.ENSP00000331746

UniGene: Hs.37058
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