Product Details

Uniprot No.

P31944

Target Names

CASP14

Alternative Names

Apoptosis related cysteine protease antibody; CASP 14 antibody; CASP-14 antibody; CASP14 antibody; Caspase 14 apoptosis related cysteine protease antibody; Caspase 14 precursor antibody; Caspase-14 subunit p10 antibody; Caspase14 antibody; CASPE_HUMAN antibody; MGC119078 antibody; MGC119079 antibody; MICE antibody; Mini ICE antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human CASP14

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9B10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Caspase-14 occupies a unique position within the caspase family, functioning primarily in terminal keratinocyte differentiation rather than classical apoptotic pathways. This protease plays an essential role in skin barrier formation, processing profilaggrin during cornification to generate natural moisturizing factors that maintain epidermal integrity. Its restricted expression pattern in differentiating keratinocytes makes CASP14 a valuable marker for studying skin development, barrier function disorders, and epithelial differentiation programs.

This recombinant monoclonal antibody, clone 9B10, offers the reproducibility and consistency that demanding research applications require. Developed using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human CASP14, provides reliable target recognition across multiple experimental platforms.

Validation studies confirm robust performance in western blotting, immunohistochemistry, and flow cytometry applications. Western blot analysis of A549 whole cell lysate demonstrates specific detection at the expected molecular weight range of 28-30 kDa, consistent with the predicted 28 kDa size. Immunohistochemical staining of paraffin-embedded human skin tissue reveals clear visualization of CASP14 in its physiologically relevant context. Flow cytometry analysis using permeabilized MCF-7 cells shows distinct positive signal separation from isotype control, enabling quantitative assessment of intracellular CASP14 expression.

The antibody arrives in a stabilized liquid format with glycerol for convenient long-term storage at -20°C or -80°C. Researchers investigating epidermal differentiation, skin barrier pathologies, ichthyosis, atopic dermatitis, or keratinocyte biology will find this antibody well-suited for characterizing CASP14 expression across diverse experimental workflows.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum. Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin. In vitro has a preference for the substrate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF. Involved in processing of prosaposin in the epidermis. May be involved in retinal pigment epithelium cell barrier function. Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD.

Gene References into Functions

High CASP14 expression is a marker of breast cancer aggressiveness in association with proliferation, TNBC phenotype, and cancer stemness. PMID: 28570747
overexpression of S100A7 in A431 skin squamous carcinoma cells significantly promoted cell proliferation in vitro and tumor growth in vivo, whereas it suppressed the expression of GATA-3 and caspase-14 PMID: 25651379
caspase-14 contributes to retinal pigment epithelium cell barrier disruption under hyperglycemic conditions. PMID: 25121097
Caspase-14 was decreased in inflammatory lesions compared to non-lesion in atopic dermatitis. The amount of caspase-14 in the lesions correlated with clinical severity as determined by eczema area and severity index score and the skin barrier functions. PMID: 25315296
Mesotrypsin generated saposins A-D from prosaposin, and mature caspase-14 contributed to this process by activating mesotrypsinogen to mesotrypsin. Knockdown of these proteases markedly down-regulated saposin A synthesis in skin equivalent models. PMID: 24872419
partial loss of caspase 14 is not associated with dedifferentiation in neoplastic lesions of the oral mucosa PMID: 23645350
genetic polymorphisms in AICDA and CASP14 are associated with risk for brain tumor in Korean children. PMID: 23408445
Suggest caspase-14 is a marker of human skin differentiation during development. PMID: 23377137
Results suggest that caspase-14 may interact with GCM1 to participate in syncytiotrophoblast differentiation during placental development. PMID: 23580611
ceramides, an important structural lipid, stimulate caspase-14 expression, coordinating formation of lipid lamellar membranes with the formation of corneocytes PMID: 23362869
Caspase-14 might play a significant role in the pathogenesis of diabetic retinopathy by accelerating retinal endothelial and epithelial cells death. PMID: 22876114
regulation of procaspase-14 maturation during terminal differentiation is a unique two-step process involving KLK7 and an activation intermediate of caspase-14. PMID: 22825846
genetic polynorphism is associated with the risk of childhood leukemia PMID: 22548721
A strong association was evident between GATA-3 and caspase-14 expression in preinvasive ductal carcinoma in situ samples, where GATA-3 also displayed prognostic significance PMID: 21930782
Regulation of caspase 14 levels provides a possible link between impaired skin barrier function and inflammatory reactions in skin diseases such as atopic dermatitis and may offer an explanation to the skin barrier dysfunction in inflamed skin lesions. PMID: 21539619
Casp-14 overexpression correlated with tumor stage, cell differentiation and lymphovascular involvement, suggesting that casp-14 was associated with tumor cell growth and metastatic potential. PMID: 21567094
Caspase 14 is a cysteine protease and is inhibited by full-length LEKTI and 5 recombinant fragments of LEKTI to varied extents. PMID: 20533828
Purification and characterization of active caspase-14 from human epidermis and development of the cleavage site-directed antibody PMID: 19960512
Results imply that caspase-14 inhibits trophoblast differentiation. PMID: 19747408
processing of caspase 14 in epidermal differentiation PMID: 12200134
caspase-14, similarly to other keratinocyte differentiation-associated proteins, is downregulated by retinoids, suggesting that this caspase plays a part in terminal keratinocyte differentiation and skin barrier formation PMID: 12445205
Loss of caspase-14 expression is associated with psoriatic lesions PMID: 15331408
psoriatic keratinocytes may activate mechanisms that prevent the nuclear entry of caspase 14 PMID: 15619438
caspase-14 is aberrantly expressed in epithelial tumors PMID: 16061209
there are tumor-specific alterations in caspase-14 expression which may define subsets of epithelial cancers with distinct clinical behaviors PMID: 16061862
Caspase-14 is present in the human placenta, primarily in the trophoblast, but its function is not clear PMID: 16168224
caspase-14 has a substrate specificity similar to the group I caspases, and demonstrate that it functions in a distinct manner from executioner caspases to carry out specific proteolytic events during keratinocyte differentiation. PMID: 16854378
caspase-14 is up-regulated during trophoblast differentiation, as represented by the BeWo cell line PMID: 17359582
Expression of exogenous caspase-14 led to growth inhibition and reduced the tumorigenicity of A431 skin cancer cells. PMID: 17436577
caspase-14 gene is rarely mutated in colorectal carcinomas, but not mutated in gastric, lung, breast and hepatocellular carcinomas PMID: 17558860
These data reveal the basic organization of the human caspase-14 promoter and suggest an important role of AP-1 and NFkappaB in the transcriptional control of caspase-14. PMID: 18424262
Expression of caspase 6 and caspase 14 genes were different between normal skin of keloid-prone individuals and normal skin of keloid-resistant patients. PMID: 18762957

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Involvement in disease

Ichthyosis, congenital, autosomal recessive 12 (ARCI12)

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Peptidase C14A family

Tissue Specificity

Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only exp

Database Links

HGNC: 1502

OMIM: 605848

KEGG: hsa:23581

STRING: 9606.ENSP00000221740

UniGene: Hs.466057

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Code	CSB-RA555705A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A549 whole cell lysate(20µg) All lanes: Caspase 14 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 28 kDa Observed band size: 28-30 kDa Exposure time：1min30s IHC image of CSB-RA555705A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA555705A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CASP14 Recombinant Monoclonal Antibody

Product Details

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Target Background