CCNH Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA091191A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: SW620 whole cell lysate(30µg),HEK293 whole cell lysate(30µg), Raji whole cell lysate(30µg), Hela whole cell lysate(30µg),A549 whole cell lysate(30µg),K562 whole cell lysate(30µg),Mouse small intestine tissue lysate(30µg) All lanes: Cyclin H antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time：2min IHC image of CSB-RA091191A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of THP-1 cell with CSB-RA091191A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing PC-3 cells stained with CSB-RA091191A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

P51946

Target Names

CCNH

Alternative Names

6330408H09Rik antibody; AI661354 antibody; AV102684 antibody; AW538719 antibody; CAK antibody; CAK complex subunit antibody; ccnh antibody; CCNH_HUMAN antibody; CDK activating kinase antibody; CDK activating kinase complex subunit antibody; Cyclin dependent kinase activating kinase antibody; cyclin dependent kinase activating kinase complex subunit antibody; Cyclin H antibody; Cyclin-H antibody; CyclinH antibody; MO15 associated protein antibody; MO15-associated protein antibody; p34 antibody; p36 antibody; p37 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human CCNH

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9A1

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Cyclin H serves as an essential regulatory subunit of the CDK-activating kinase complex, playing a fundamental role in cell cycle progression and transcription regulation through its association with CDK7. This protein is critical for the phosphorylation and activation of cyclin-dependent kinases, making it a key target for researchers investigating cell cycle control, transcriptional machinery, and cancer biology.

This recombinant monoclonal antibody, developed using rabbit host immunization against a synthetic peptide derived from human CCNH, offers the reproducibility and consistency that demanding experimental workflows require. As a sequence-defined reagent produced from a single clone (9A1), it eliminates the lot-to-lot variability that can compromise longitudinal studies and ensures reliable performance across your research timeline.

Validation across multiple applications demonstrates this antibody's versatility in your experimental toolkit. Western blot analysis confirms specific detection at the expected 38 kDa molecular weight across diverse human cell lines including SW620, HEK293, Raji, HeLa, A549, and K562, with cross-species reactivity demonstrated in mouse small intestine tissue. Immunohistochemistry validation in human testis tissue reveals clear staining patterns, while immunofluorescence studies in THP-1 cells show distinct nuclear localization consistent with Cyclin H's known function. Flow cytometry analysis in PC-3 cells further confirms the antibody's utility for single-cell applications, with clear separation from isotype control.

The unconjugated format and affinity-purified preparation provide flexibility for pairing with your preferred detection systems. Whether you're exploring CDK7 complex assembly, investigating transcription-coupled DNA repair mechanisms, or studying cell cycle dysregulation in disease models, this antibody delivers the specificity and cross-species reactivity needed for comprehensive Cyclin H research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Gene References into Functions

Expressions of components of the CAK complex, CDK7, MAT1, and Cyclin H are elevated in breast cancer. PMID: 27301701
Our results indicated that CCNH/CDK7-CtBP2 axis may augment ESCC cell migration, and targeting the interaction of both may provide a novel therapeutic target of esophageal squamous cell carcinoma . PMID: 25820824
SNPs in CCNH and ABCG2 can modulate the development of severe oxaliplatin-induced peripheral neuropathy. PMID: 24351404
Hemodynamic forces modulate EC proliferative phenotype through the miR-23b/CAK/cyclin H pathway. PMID: 24855060
When considering all thyroid cancer (DTC) cases, only rs2230641 (CCNH) was associated with DTC risk. PMID: 23982724
CCNH and CDK7 may play an important role in the tumorigenesis and development of esophageal squamous cell carcinoma. PMID: 23456497
High Cyclin H is associated with gastrointestinal stromal tumours. PMID: 20598140
The expression of cyclin H and CDK7 protein in proliferating hemangiomas was significantly higher than that in involuting hemangiomas and normal skin tissues. PMID: 18950027
The cyclin H/cdk7/Mat1 kinase activity is regulated by CK2 phosphorylation of cyclin H. PMID: 12140753
Confocal microscopy revealed the co-localization of PKC-iota with CAK/cdk7 in both the cytoplasm and nucleus of U-373 MG glioma cells, supporting its role in cell signaling PMID: 15695176
In response to ATRA, PML/RARalpha is dissociated from CAK, leading to MAT1 degradation, G1 arrest, and decreased CAK phosphorylation of PML/RARalpha PMID: 16935935
Retinoic-acid-induced RAR-CAK signaling events appear to proceed intrinsically during granulocytic development of normal primitive hematopoietic cells. ALDH-governed RA availability may mediate this process by initiating RAR-CAK signaling. PMID: 17628022
These results suggested that genetic variants in CAK genes, Cdk7, cyclin H, MAT1, might modulate the risk of lung cancer in a gene-gene interaction mode, which consist to the biochemical interaction of corresponding proteins. PMID: 17707548
Our study demonstrates the independent prognostic value of cyclin H expression in diffuse large B-cell lymphoma and proposes its use as a prognostic marker. PMID: 18400256
TFIIH changes subunit composition in response to DNA damage. The CAK is released from the core during nucleotide excision repair (NER). PMID: 18614043

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Subcellular Location

Nucleus.

Protein Families

Cyclin family, Cyclin C subfamily

Database Links

HGNC: 1594

OMIM: 601953

KEGG: hsa:902

STRING: 9606.ENSP00000256897

UniGene: Hs.292524

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cell cycle DNA repair pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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