CENPC Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA952093A0HU
Size	US$210
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Image	Western Blot Positive WB detected in:JURKAT whole cell lysate(30µg), HL-60 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: CENPC antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 107 kDa Observed band size: 120 kDa Exposure time：1min Overlay Peak curve showing jurkat cells stained with CSB-RA952093A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q03188

Target Names

CENPC

Alternative Names

CENP-C 1 antibody; CENP-C antibody; CENPC_HUMAN antibody; CENPC1 antibody; Centromere autoantigen C antibody; Centromere autoantigen C1 antibody; Centromere protein C 1 antibody; Centromere protein C antibody; hcp-4 antibody; ICEN7 antibody; Interphase centromere complex protein 7 antibody; MIF2 antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human CENPC

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4E2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CENPC (Centromere Protein C) serves as a fundamental component of the constitutive centromere-associated network, playing an essential role in kinetochore assembly and chromosome segregation during cell division. This inner centromere protein directly links centromeric chromatin to the outer kinetochore machinery, making it a critical target for researchers investigating mitotic fidelity, chromosome instability, and the molecular basis of centromere function.

This recombinant monoclonal antibody, clone 4E2, offers the reproducibility and consistency that demanding centromere research requires. Developed using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit host and monoclonal nature ensure high specificity against human CENPC, with cross-species reactivity extending to mouse samples.

Western blot validation demonstrates reliable detection across multiple cell lines, including human JURKAT and HL-60 cells as well as mouse NIH/3T3 cells, confirming functional cross-species reactivity. The observed band at approximately 120 kDa runs slightly higher than the predicted 107 kDa molecular weight, a shift commonly attributed to post-translational modifications such as phosphorylation, which is known to regulate CENPC function during the cell cycle. Flow cytometry validation in JURKAT cells confirms utility for intracellular detection of this nuclear protein, with clear separation from isotype control signals.

The antibody's flexibility across ELISA, western blot, and flow cytometry applications supports diverse experimental workflows, from biochemical characterization to single-cell analysis. Researchers studying mitotic regulation, aneuploidy mechanisms, or centromere biology will find this antibody a dependable tool for investigating CENPC dynamics and function.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENPC recruits DNA methylation and DNMT3B to both centromeric and pericentromeric satellite repeats and regulates the histone code in these regions.

Gene References into Functions

Whereas CENP-C recruits a single MIS12:NDC80 complex, the authors show here that CENP-T binds one MIS12:NDC80 and two NDC80 complexes upon phosphorylation by the mitotic CDK1:Cyclin B complex at three distinct CENP-T sites. PMID: 28012276
CENP-C specifically binds alpha satellite non-coding RNAs. PMID: 28787590
A nonhistone centromere protein, CENP-C, binds and reshapes the nucleosome, sliding the DNA gyres back to positions similar to those in canonical nucleosomes containing conventional histone H3. PMID: 26878239
HPV18 E7 inhibited the binding of CENP-C to alpha-satellite DNAs. PMID: 25997930
CENP-C recruits the Ndc80 complex through its interactions with KNL1 and the Mis12 complex at kinetochores during chromosome segregation. PMID: 25660545
CENP-C, a CCAN subunit, is crucial for kinetochore assembly because it links centromeres with the microtubule-binding interface of kinetochores. PMID: 26124289
CENP-C affects nucleosome shape and dynamics in a manner analogous to allosteric regulation of enzymes. CENP-C depletion leads to rapid removal of CENP-A from centromeres, indicating their collaboration in maintaining centromere identity. PMID: 25954010
CENP-C works as an important factor for centromeric M18BP1 recruitment PMID: 22540025
Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore. PMID: 21353556
CENP-C may target the centromere by establishing multiple contacts with both the DNA and protein constituents of the kinetochore PMID: 12490152
CENPC target sites that can be sumoylated by SUMO-2 were shown to be equally susceptible to SUMO-1 attachments which include specific sites on SUMO-2 itself, Ubc9, and the recombinant CENP-C fragments PMID: 15272016
a functional interplay between the chromosomal passenger complex and CENP-C. PMID: 17287400
Using protein truncation and in vitro mutagenesis, we have identified the nucleolar localization sequences on CENPC1,and evidence shows that CENPC1 is an RNA-associating protein that binds alpha-satellite RNA by an in vitro binding assay. PMID: 17623812
CENP-C and CENP-H co-localize to the CENP-A chromatin domain. PMID: 17651496
DNMT3B interacts with CENP-C to modulate DNA methylation and the histone code at centromere. PMID: 19482874
C-terminal domain of CENP-C displays multiple and critical functions for mammalian centromere formation PMID: 19503796

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Subcellular Location

Nucleus. Chromosome, centromere, kinetochore. Chromosome, centromere.

Protein Families

CENP-C/MIF2 family

Database Links

HGNC: 1854

OMIM: 117141

KEGG: hsa:1060

STRING: 9606.ENSP00000273853

UniGene: Hs.479867

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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