Product Details

Uniprot No.

Target Names

CKB

Alternative Names

B CK antibody; B-CK antibody; BB-CK antibody; BCK antibody; Brain creatine kinase antibody; Ckb antibody; CKBB antibody; Creatine kinase B antibody; Creatine kinase B chain antibody; Creatine kinase B type antibody; Creatine kinase B-type antibody; Creatine Kinase BB Isoenzyme antibody; Creatine kinase brain antibody; Creatine kinase brain type antibody; Creatine phosphokinase BB antibody; Epididymis luminal protein 211 antibody; Epididymis secretory protein Li 29 antibody; HEL 211 antibody; HEL S 29 antibody; KCRB_HUMAN antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human CKB

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

3E4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Creatine kinase B-type (CKB) serves as the predominant cytoplasmic isoenzyme responsible for energy homeostasis in tissues with high and fluctuating energy demands, particularly the brain. By catalyzing the reversible transfer of phosphate between ATP and creatine, CKB maintains cellular energy reserves critical for neuronal function, making it an essential marker in neuroscience research, metabolic studies, and investigations of brain-specific pathologies.

This recombinant monoclonal antibody, generated in rabbit against a synthetic peptide derived from human CKB, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Unlike traditional hybridoma-derived antibodies, recombinant production ensures lot-to-lot uniformity, allowing researchers to maintain confidence in their results across extended studies and collaborative projects.

Validation studies demonstrate robust performance across multiple applications and species. In Western blot analysis, the antibody successfully detects CKB in human cell lines including HEK293, HeLa, and PC-3, as well as in mouse and rat brain tissue lysates, confirming reliable cross-species reactivity for comparative studies. The observed band size of approximately 48 kDa, slightly higher than the predicted 43 kDa molecular weight, likely reflects post-translational modifications characteristic of the native protein. Flow cytometry validation using HeLa cells shows clear separation between specific staining and isotype control populations, supporting intracellular detection applications.

With validated performance in ELISA, Western blot, and flow cytometry, this antibody provides flexibility for researchers investigating CKB expression in neurological disease models, cellular energy metabolism, or tissue-specific creatine kinase distribution studies. The confirmed cross-species reactivity across human, mouse, and rat samples makes it particularly valuable for translational research requiring consistent detection across preclinical models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work.

Gene References into Functions

The data revealed a varying but significant increase of CK activity in CKBE individuals as compared to controls, reaching an almost 800-fold increase in two CKBE individuals which also had increased erythrocyte creatine. PMID: 28364583
Association and oligomerization of Prx II could take part in recovery and protection of the CK BB enzyme activity from inactivation during heat-induced stress. PMID: 29227081
membrane localization of BCK seems to be an important and regulated feature for the fueling of membrane-located, ATP-dependent processes, stressing again the importance of local rather than global ATP concentrations. PMID: 27318991
These findings support increased CK activity as protection against ischaemia-reperfusion injury, in particular, protection via CKMT2 in a cardiac-relevant cell line, which merits further investigation in vivo. PMID: 28806770
SRC, LYN and CKB expression or DNA methylation could be useful markers for predicting tumor progression. PMID: 26460485
CK-MB levels were higher after ERCP in non-ischemic patients compared with a myocardial ischemia group. Creatine phosphokinase levels did not differ significantly between groups. PMID: 25141318
CK-B catalytic activity also helps in the formation of protrusive ruffle structures which are actin-dependent and abundant on the surface of both unstimulated and LPS-activated macrophages. PMID: 25538032
increased serum Ckbb reflects failure of osteoclasts or suppression of osteoclasts in children with osteogenesis imperfecta during neridronate treatment PMID: 24518563
Estimation of CK and its CK isoenzyme fractions can aid in quick and accurate diagnosis of tubal ectopic pregnancy. PMID: 23876027
effects of osmolytes on human brain-type creatine kinase folding PMID: 22885020
We found that most patients with macular telangiectasia-2 possess retinal autoantibodies, the most prevalent of which were directed against AGL, RBP3, and CK-B. PMID: 23882694
analysis of SNPs and their effect on creatine kinase structure and function PMID: 23049898
study found promoter SNPs of CKB and TPI1 were weakly associated with schizophrenia;in addition, IFNG polymorphisms were associated with schizophrenia; results suggest that IFNG and proteins affected by IFNG may play a role in the pathogenesis of schizophrenia PMID: 22623148
de-methylated CKB gene is inherited that leads to high CKB expression levels in myeloic precursor cells in the bone marrow PMID: 22088263
His103 and Phe107 in hASB9-2 are essential for binding to CKB. PMID: 22418839
Cigarette smoke induced carbonylation and subsequent degradation of creatine kinase B are involved in the regulation of senescence in bronchial epithelial cells. PMID: 21980054
The results suggested that the intra- and inter-subunit domain interactions modified the behavior of kinetic refolding. PMID: 21931810
Data demonstrate that the downregulation of CKB may play an important role in colon cancer progression by promoting EMT. PMID: 21308735
CKB was up-regulated in women older than 38 years, and its expression in cumulus cells was associated with embryo quality PMID: 20721618
These observations suggested that the ability to generate the oxidized form could protect BBCK against the intracellular oxidative stress. PMID: 20923681
Report the effects of prior calcium channel blocker therapy on creatine kinase-MB (myocardial form) levels after percutaneous coronary interventions. PMID: 19337554
A single troponin I value at 3 days from symptom onset is a better predictor of infarct size compared to peak values and CK-MB. PMID: 20588136
Phosphocreatine metabolism in the normal appearing white matter in multiple sclerosis is impaired due to decreased CK-B levels. PMID: 20520825
The correlation of CK-BB as a disease biomarker in both CNS and peripheral tissues from Huntington's disease mice and patients may provide a powerful means to assess disease progression and prognosis. PMID: 20460152
2D fingerprinting & mass spectrometry reveal specific targets of protein oxidation in Alzheimer's disease brain, including creatine kinase BB, suggesting involvement of oxidatively modified proteins in neurodegeneration. PMID: 12160938
Expression of CKB mRNA and CK-B sometimes occurred in blastic transformation of the hematopoietic system PMID: 15996648
CKB was expressed in 78% of colon tumors PMID: 16424007
GM130 and BB-CK co-localize specifically in a transient fashion during early prophase of mitosis, when GM130 plays an important role in Golgi fragmentation that starts also at early prophase. PMID: 17036164
The asymmetric unit contained two molecules of CKB, giving a crystal volume per protein mass (Vm) of 1.80 A3 Da-1 and a solvent content of 31.6%. PMID: 18309274
Autoantibodies to EsteD and BB-CK produced in experimental autoimmune uveoretinitis -induced mice were also detected in some endogenous uveitis patients, suggesting that these proteins might be autoantigens. PMID: 18552983
Using a yeast 2-hybrid it was discovered that the C-terminal domain of KCC3, that is lost in most hereditary motor and sensory neuropathy with agenesis of the corpus callosum-causing mutations, directly interacts with brain-specific creatine kinase. PMID: 18566107
The Tat-CK fusion protein markedly increased endogenous CK activity levels within PC12 cells. PMID: 18682038
Three structural aspects of human-brain-type-creatine-kinase were identified by X-ray crystallography: the ligand-free-form at 2.2A; the ADP-Mg2+, nitrate, and creatine complex (transition-state-analogue complex; TSAC); and the ADP-Mg2+-complex at 2.0A. PMID: 18977227
Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527

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Subcellular Location

Cytoplasm, cytosol. Mitochondrion.

Protein Families

ATP:guanido phosphotransferase family

Database Links

HGNC: 1991

OMIM: 123280

KEGG: hsa:1152

STRING: 9606.ENSP00000299198

UniGene: Hs.173724

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Code	CSB-RA936751A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: HEK293 whole cell lysate(30µg),Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg),Mouse brain tissue lysate(30µg),Rat brain tissue lysate(30µg) All lanes: Creatine kinase B type antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 43 kDa Observed band size: 48 kDa Exposure time：2min Overlay Peak curve showing Hela cells stained with CSB-RA936751A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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CKB Recombinant Monoclonal Antibody

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Target Background