Code | CSB-RA217204A0HU |
Size | US$210 |
Order now | |
Image | |
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
To produce a recombinant monoclonal antibody against COL17A1, CUSABIO initiated the process by immunizing a rabbit with a synthesized peptide corresponding to the human COL17A1 protein. Subsequent steps involved isolating B cells from the rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which was employed as a template for amplifying COL17A1 antibody genes using degenerate primers. These amplified COL17A1 antibody genes were then integrated into a plasmid vector and introduced into host cells for expression. The COL17A1 recombinant monoclonal antibody was purified from the cell culture supernatant through affinity chromatography and evaluated for its utility in ELISA and WB applications, with specificity demonstrated for human COL17A1 protein.
COL17A1 is a critical structural protein that contributes to the stability of the skin and mucous membranes. Its role in anchoring the epidermis to the basement membrane, mediating cell-ECM adhesion, and maintaining the epidermal barrier is essential for the structural integrity and function of these tissues.
There are currently no reviews for this product.