COX6B1 Recombinant Monoclonal Antibody

COX6B1 is a nuclear-encoded subunit of cytochrome c oxidase, the terminal enzyme complex of the mitochondrial respiratory chain. As a key component of Complex IV, COX6B1 plays an essential role in oxidative phosphorylation and cellular energy metabolism. Mutations in this gene have been linked to mitochondrial disorders, making it a relevant target for researchers investigating mitochondrial function, metabolic diseases, and cellular bioenergetics.

This recombinant monoclonal antibody against human COX6B1 offers the reproducibility and consistency that demanding experimental workflows require. Generated from a defined sequence and produced as a rabbit IgG clone (11F2), it eliminates the lot-to-lot variability inherent in traditional polyclonal preparations, ensuring your results remain comparable across extended studies.

Validation testing demonstrates reliable performance across multiple platforms. In western blot applications, the antibody detects a band at the predicted 10 kDa molecular weight across diverse human cell lines including MCF7, COLO205, A549, HepG2, and HeLa, confirming specificity for the target protein. For tissue-based studies, immunohistochemistry has been validated in paraffin-embedded human brain and liver cancer sections using citrate buffer antigen retrieval, providing clear visualization of COX6B1 expression patterns. Flow cytometry analysis in HeLa cells further extends its utility for quantitative single-cell studies of mitochondrial protein expression.

The antibody is supplied unconjugated in a glycerol-containing buffer optimized for long-term storage stability. Whether you are characterizing mitochondrial dysfunction in disease models, profiling metabolic markers across cell populations, or investigating respiratory chain assembly, this antibody provides a dependable tool for your COX6B1 detection needs.