Product Details

Uniprot No.

Target Names

CSE1L

Alternative Names

Exportin-2 (Exp2) (Cellular apoptosis susceptibility protein) (Chromosome segregation 1-like protein) (Importin-alpha re-exporter), CSE1L, CAS XPO2

Species Reactivity

Human

Immunogen

A synthesized peptide from human CSE1L protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5D10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

CSE1L, also known as Exportin-2 or Cellular Apoptosis Susceptibility protein, plays a central role in nucleocytoplasmic transport by mediating the re-export of importin-alpha from the nucleus back to the cytoplasm. This function is essential for maintaining the nuclear transport cycle that governs protein localization and cellular homeostasis. Beyond its transport role, CSE1L has garnered significant research attention for its involvement in chromosome segregation, cell proliferation, and apoptosis regulation, making it a compelling target in cancer biology and cell cycle research.

This recombinant monoclonal antibody, clone 5D10, offers the consistency and reliability that demanding experimental workflows require. Produced using recombinant technology with a sequence-defined approach, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring reproducible results across long-term studies and multi-site collaborations.

Validation data demonstrate robust performance across multiple applications. In immunohistochemistry, the antibody produces clear staining in paraffin-embedded human testis tissue at dilutions of 1:50–1:200, with antigen retrieval in citrate buffer at pH 6.0. Immunofluorescence studies in A431 cells reveal distinct signal when counterstained with DAPI, while flow cytometry analysis of BxPC-3 cells shows a clear positive shift compared to isotype control, confirming specific detection of intracellular CSE1L.

The antibody's versatility across IHC, immunofluorescence, and flow cytometry provides researchers with flexibility to investigate CSE1L expression and localization using complementary techniques. Whether exploring nuclear transport mechanisms, examining CSE1L's role in tumor progression, or investigating its contribution to apoptotic pathways, this antibody delivers the specificity and reproducibility essential for meaningful experimental outcomes.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Gene References into Functions

CSE1L expression was correlated with MSH6 expression in tumor samples and was associated with poor prognosis in patients with osteosarcoma. Taken together, our results demonstrate that the CSE1L-MSH6 axis has an important role in osteosarcoma progression. PMID: 28387323
CSE1L appears to be critical for the nuclear import of certain key repressive proteins. Indeed, NOVA1, HDAC1, HDAC2, and HDAC8, genes known as silencing factors, became delocalized into cytosol upon CSE1L depletion. PMID: 29636421
The combination of CTNB1, XPO2, and CAPG achieved 95% sensitivity and 96% specificity for the discrimination of these subtypes. We developed two uterine aspirate-based signatures to diagnose Endometrial cancer and classify tumors in the most prevalent histologic subtypes. This will improve diagnosis and assist in the prediction of the optimal surgical treatment PMID: 28790116
Our data suggest a previously unanticipated link between CAS and integrin beta1 signaling which correlates with an aggressive hepatocellular carcinoma phenotype. PMID: 27015362
CSE1L knockdown by shRNA inhibited protein, resulting in decreased cell proliferation, reduced colony formation in soft agar, and induction of apoptosis. CSE1L protein is expressed early and across all stages of colorectal carcinoma (CRC) development. shRNA knockdown of CSE1L was associated with inhibition of tumorigenesis in CRC cells. CSE1L may represent a potential target for treatment of CRC. PMID: 27521996
Data suggest that CAS overexpression in thyroid carcinoma depends on the subtype and the disease stage. Our findings also indicate that CAS maintains papillary thyroid cell proliferation and survival. PMID: 26892809
There is a relationship between nuclear CSE1L overexpression and distant metastasis in breast cancer. PMID: 26278417
CAS plays contrasting roles in proliferation and apoptosis PMID: 26668314
hCAS/CSE1L is responsible for controlling the homologous recombinational repair activity by directly interacting with RAD51. PMID: 26123175
nuclear CSE1L is mainly non-phosphorylated CSE1L and is involved in gene regulation and cytoplasmic CSE1L is mainly phosphorylated CSE1L and is involved in cytoplasmic signaling regulation in melanocytic tumorigenesis. PMID: 25973023
These results indicate that CSE1L is associated with viability and apoptosis, cellular adhesion and invasion, thus implicating CSE1L in the progression of colorectal cancer. PMID: 22450763
The cellular apoptosis susceptibility/importin-alpha1 transport cycle is linked to X-linked inhibitor (XIAP) and is required to maintain tumor cell survival in hepatocellular carcinoma. PMID: 24799195
CSE1L expression was significantly inhibited in RKO cells, causing cell cycle arrest in the G2/M and S phases and a delay in cell proliferation, as well as induction of apoptosis and an inhibition of colony growth capacity. PMID: 23621178
Most colorectal tumors were positive for CSE1L staining (98.4%). CRT's with K-Ras mutation or high cytoplasmic CSE1L expression were correlated with T status (depth of tumor penetration; P = .004), stage (P = .004), and lymph node metastasis (P = .019). PMID: 23806821
Our results suggest that urinary CSE1L deserves further evaluation for the screening of bladder cancer. PMID: 22653741
CSE1L- and inhibitor of DNA binding-3 (ID3)-overexpression was associated with the diagnosis of BL and signal transduction and transcription-3 (STAT3) with DLBCL (P<0.001 for all markers). All three markers were associated with patient outcome in DLBCL PMID: 22967991
CSE1L may be involved in the "early" and "late" metastasis of tumor cells in tumorigenesis. PMID: 22952058
Nuclear CSE1L may play an oncogenic role in bladder tumor progression and that immunohistochemical staining of nuclear CSE1L may be useful for the prognosis of bladder urothelial carcinomas. PMID: 22476051
CAS protein expression is related closely to tumor differentiation in hepatocellular carcinoma tissues. PMID: 23189846
Data show that CSE1L, DIDO1 and RBM39 mRNA expression levels correlated with chromosome 20q DNA copy number status. PMID: 22711543
the requirement for and the regulation of CAS in the functioning of the Vpr-Impalpha complex PMID: 22110766
Increased immunoexpression of CAS protein in serous ovarian tumors may be useful in identifying the patients with more aggressive disease. PMID: 21290345
These results indicate that examination of CSE1L and E-cadherin distribution in colorectal epithelium glands may be valuable for evaluating the malignance of colorectal disease. PMID: 20734115
Serum cellular apoptosis susceptibility protein may have a role in progression of metastatic colorectal cancer PMID: 20150437
CAS/CSE 1 stimulates E-cadhrin-dependent cell polarity in HT-29 human colon epithelial cells. PMID: 12061792
CSE1L/CAS has a role in proliferation and apoptosis [review] PMID: 12510150
a single phosphorylation site on CAS can effectively separate cell migration from other transformed growth characteristics PMID: 12972425
Results suggested that CAS may be associated with cell proliferation rather than apoptosis, and further, CAS might play an important role in the development of human hepatocellular carcinoma. PMID: 16786158
hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. PMID: 17719542
These results indicate that CAS may play an important role in regulating the cytotoxicities of chemotherapeutic drugs. PMID: 18377724
Results show that heat upregulates the initial docking of importin-alpha at the nuclear envelope and stimulates the translocation of cellular apoptosis susceptibility protein into the nuclear interior. PMID: 18425415
PHAPI, CAS, and Hsp70 function together to accelerate nucleotide exchange on Apaf-1 and prevent inactive Apaf-1/cytochrome c aggregation. PMID: 18439902
Herein, we report that cellular apoptosis susceptibility (CAS) (or CSE1L) protein regulates the secretion of HT-29 human colorectal cells. PMID: 19224336

Hide All

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

XPO2/CSE1 family

Tissue Specificity

Detected in brain, placenta, ovary, testis and trachea (at protein level). Widely expressed. Highly expressed in testis and in proliferating cells.

Database Links

HGNC: 2431

OMIM: 601342

KEGG: hsa:1434

STRING: 9606.ENSP00000262982

UniGene: Hs.90073

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Code	CSB-RA901089A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA901089A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of A431 cell with CSB-RA901089A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing BxPC-3 cells stained with CSB-RA901089A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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