Code | CSB-RA153677A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The CYP3A4 recombinant monoclonal antibody is well-suited for detecting human CYP3A4 protein in ELISA and IHC applications. Its production is accomplished through the use of recombinant DNA technology, which involves synthesizing the gene that codes for the CYP3A4 monoclonal antibody following the sequencing of the cDNA of the CYP3A4 antibody-producing hybridomas. To produce these hybridomas, B cells isolated from animals immunized with a synthesized peptide derived from human CYP3A4 are fused with myeloma cells. The synthesized gene is then cloned into a vector and transfected into cells for cultivation. The resulting CYP3A4 recombinant monoclonal antibody is purified via affinity chromatography from the cell culture supernatant.
The CYP3A4 protein, also known as cytochrome P450 3A4, is a member of the cytochrome P450 family of enzymes, which play a crucial role in the metabolism of various drugs, toxins, and endogenous compounds in the liver and other tissues. Specifically, CYP3A4 is responsible for the metabolism and breakdown of a large number of drugs, including statins, anti-cancer drugs, anti-depressants, anti-arrhythmic drugs, and many others. It is also involved in the metabolism of steroid hormones, such as testosterone, estrogen, and cortisol.
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