Product Details

Uniprot No.

P35659

Target Names

DEK

Alternative Names

Protein DEK, DEK

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human DEK

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

20D2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DEK is a chromatin-associated protein that plays essential roles in transcriptional regulation, DNA repair, and mRNA splicing. As an autoantigen frequently targeted in autoimmune conditions and a protein implicated in various malignancies, DEK has become an important focus for researchers investigating epigenetic mechanisms and nuclear signaling pathways. Its involvement in chromatin architecture and gene expression regulation makes it particularly relevant for cancer biology and autoimmunity studies.

This recombinant monoclonal antibody, clone 20D2, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human DEK and produced using recombinant technology, this antibody provides sequence-defined specificity with minimal lot-to-lot variation. The rabbit IgG format ensures reliable performance across multiple experimental platforms while maintaining the sensitivity needed for detecting this nuclear protein.

Validation studies demonstrate robust performance across several key applications. Immunohistochemistry testing in paraffin-embedded human gastric cancer and breast cancer tissues at 1:50 dilution reveals clear nuclear staining patterns consistent with DEK's known localization, making this antibody well-suited for pathology-focused investigations. Immunofluorescence studies in HeLa cells confirm nuclear detection with excellent signal clarity when counter-stained with DAPI. Flow cytometry analysis, also performed in HeLa cells, shows distinct positive population shifts compared to isotype controls, enabling quantitative assessment of DEK expression at the single-cell level.

The antibody is supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C. With validated applications spanning tissue-based analysis, cellular imaging, and flow cytometry, this antibody supports diverse experimental workflows for researchers exploring DEK's contributions to chromatin biology, oncogenesis, and nuclear signaling mechanisms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA796958A0HU
Size	US$210
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Image	IHC image of CSB-RA796958A0HU diluted at 1:50 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.67% DAB. IHC image of CSB-RA796958A0HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.67% DAB. Immunofluorescence staining of Hela with CSB-RA796958A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 524-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA796958A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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DEK Recombinant Monoclonal Antibody

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