Code | CSB-RA796958A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
FC | 1:50-1:200 |
To produce a recombinant monoclonal antibody against DEK, CUSABIO initiated the process by immunizing a rabbit with a synthesized peptide corresponding to human DEK. Following immunization, B cells were isolated from the rabbit, and RNA was extracted from these cells. The extracted RNA was reverse-transcribed into cDNA, which was utilized as a template for extending DEK antibody genes using degenerate primers. These extended DEK antibody genes were then integrated into a plasmid vector and introduced into host cells for expression. The DEK recombinant monoclonal antibody was purified from the cell culture supernatant through affinity chromatography and evaluated for its utility in ELISA, IHC, IF, and FC applications, with specificity demonstrated for human DEK protein.
DEK is a nuclear protein that has been implicated in both normal cellular functions and disease-related processes. It is known to play various roles in the cell nucleus and is associated with several cellular processes, including chromatin organization, DNA binding, transcription regulation, cell cycle regulation, and immune response.
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