EHD1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA547153A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: SW620 whole cell lysate(30µg), A-431 whole cell lysate(30µg), A549 whole cell lysate(30µg), HeLa whole cell lysate, NIH/3T3 whole cell lysate(30µg), Mouse brain tissue lysate(30µg) All lanes: EHD1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3min IHC image of CSB-RA547153A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing PC-3 cells stained with CSB-RA547153A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q9H4M9

Target Names

EHD1

Alternative Names

CDABP0131 antibody; EH domain containing 1 antibody; EH domain-containing protein 1 antibody; EHD1 antibody; EHD1_HUMAN antibody; FLJ42622 antibody; FLJ44618 antibody; H PAST antibody; hPAST1 antibody; PAST antibody; PAST homolog 1 antibody; PAST1 antibody; Testilin antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human EHD1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13D3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

EHD1 (EH domain-containing protein 1) plays a central role in endocytic recycling, regulating the transport of internalized membrane proteins back to the cell surface. This function makes EHD1 essential for maintaining receptor homeostasis and has implicated it in diverse cellular processes including cell migration, cytokinesis, and ciliary function. Dysregulation of EHD1 has been associated with cancer progression and metastasis, positioning it as a meaningful target for researchers investigating membrane trafficking dynamics and disease mechanisms.

This recombinant monoclonal antibody, generated from clone 13D3 in rabbit host, offers the reproducibility that demanding experimental workflows require. Because the antibody sequence is defined and production occurs through recombinant expression, researchers benefit from consistent performance across lots—eliminating the variability that can complicate long-term studies or multi-site collaborations. Affinity chromatography purification ensures high specificity for your target.

Validation data demonstrates robust performance across multiple applications. Western blot analysis detects a clean band at the predicted 61 kDa molecular weight across human cell lines including SW620, A-431, A549, and HeLa, with cross-species reactivity confirmed in mouse samples including NIH/3T3 cells and brain tissue lysate. Immunohistochemistry staining has been validated in paraffin-embedded human cervical cancer tissue, revealing clear target localization. Flow cytometry analysis in PC-3 cells shows distinct positive signal separation from isotype control, enabling quantitative assessment of EHD1 expression at the single-cell level.

Whether investigating endosomal trafficking pathways, exploring EHD1's role in tumor biology, or characterizing membrane dynamics in cellular models, this antibody provides the validated, consistent performance needed for publication-quality results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis. In vitro causes vesiculation of endocytic membranes. Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth. Plays a role in myoblast fusion. Involved in the unidirectional retrograde dendritic transport of endocytosed BACE1 and in efficient sorting of BACE1 to axons implicating a function in neuronal APP processing. Plays a role in the formation of the ciliary vesicle (CV), an early step in cilium biogenesis. Proposed to be required for the fusion of distal appendage vesicles (DAVs) to form the CV by recruiting SNARE complex component SNAP29. Is required for recruitment of transition zone proteins CEP290, RPGRIP1L, TMEM67 and B9D2, and of IFT20 following DAV reorganization before Rab8-dependent ciliary membrane extension. Required for the loss of CCP110 form the mother centriole essential for the maturation of the basal body during ciliogenesis.

Gene References into Functions

Results suggest that EHD1 is a cisplatin (CDDP)-resistant gene that suppresses DNA adduct-induced apoptosis by modulating intracellular CDDP concentrations. PMID: 27411790
this study shows that overexpression of EHD1 induced the epithelial-mesenchymal transition and increased the metastatic potential of lung cancer cells in vitro and in vivo PMID: 27531895
RAB11FIP3 combines with Eps15 homology domain 1 to promote the endocytosis recycling of phosphorylation of epithelial growth factor receptor. PMID: 28215104
van der Waals interactions should be the main consideration when we design peptide inhibitors of EHD1 EH domain with high affinities. PMID: 26465136
Authors found that Eps15-homology domain 1 (EHD1), a protein that associates with the endocytic recycling compartment (ERC), colocalizes with active R-Ras in transiently expressed HeLa cells. PMID: 26378252
that Molecule Interacting with CasL Like-1 and Eps15 Homology Domain protein 1 differentially influence microtubule dynamics during early and late mitosis PMID: 25287187
Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase. PMID: 25595798
Data indicate that concordant transforming growth factor-beta1 (TGFbeta-1) positive and EH-domain containing 1 (EHD1) negative as a strong favorable prognosis factor in non-small cell lung cancer (NSCLC). PMID: 24946721
MICAL-L1-mediated recruitment of EHD1 to Src-containing recycling endosomes is required for the release of Src from the perinuclear endocytic recycling compartment in response to growth factor stimulation. PMID: 24481818
evidence that the functions of both EHD1 and EHD4 are primarily in TRE membrane vesiculation, whereas EHD3 is a membrane-tubulating protein. PMID: 24019528
the lipid modifier cPLA2alpha and EHD1 are involved in the vesiculation of CD59-containing endosomes PMID: 22456504
Rabankyrin-5 interacts with EHD1 and Vps26 to regulate endocytic trafficking and retromer function PMID: 22284051
EHD1 is involved in the control of CD59 transport from pre-sorting endosomes to the ERC in a PKC-dependent manner PMID: 20961375
A new class of cardiac trafficking proteins(EHD1, EHD2, EHD3, EHD4) regulates cardiac membrane protein targeting. PMID: 20489164
The results indicate that NPF is the preferred binding motif for the EHD1 EH-domain, but both the DPF and GPF motifs are capable of binding with lower affinity. PMID: 19798736
These data implicate MICAL-L1 as an unusual type of Rab effector that regulates endocytic recycling by recruiting and linking EHD1 and Rab8a on membrane tubules. PMID: 19864458
A tubular EHD1-containing compartment involved in the recycling of major histocompatibility complex class I molecules to the plasma membrane PMID: 12032069
EHD3: a protein that resides in recycling tubular and vesicular membrane structures and interacts with EHD1. PMID: 12121420
a novel interacting partner for EHD1, rabenosyn-5, was revealed. PMID: 15020713
ATP binding is required for oligomerization of mRme-1/EHD1, which in turn is required for its association with endosomes PMID: 15710626
Data support a role for EHD1 in beta1 integrin recycling, and demonstrate a requirement for EHD1 in integrin-mediated downstream functions. PMID: 17284518
Rab8a and Myosin Vb colocalize to a tubular network containing EHD1 and EHD3, which does not contain Rab11a. PMID: 17507647
Solution structure of the 133 C-terminal residues of EHD1, which includes the EH domain, was solved. PMID: 17899392
Results show that EHD1 undergoes serine-phosphorylation, and suggest that EHD1 phosphorylation occurs between early endosomes and the endocytic recycling compartment. PMID: 18661112
Data show that EH domain mutant (K483E) that associates exclusively with punctate membranes displayed decreased binding to phosphatidylinositol-4-phosphate and other phosphoinositides. PMID: 19369419

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Subcellular Location

Recycling endosome membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, cilium membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

TRAFAC class dynamin-like GTPase superfamily, Dynamin/Fzo/YdjA family, EHD subfamily

Tissue Specificity

Highly expressed in testis.

Database Links

HGNC: 3242

OMIM: 605888

KEGG: hsa:10938

STRING: 9606.ENSP00000320516

UniGene: Hs.523774

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Endocytosis

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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