EIF3B Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA249552A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:K562 whole cell lysate(30µg), COLO-205 whole cell lysate(30µg), A431 whole cell lysate(30µg), HEK293 whole cell lysate(30µg) All lanes: eIF3B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 92 kDa Observed band size: 116 kDa Exposure time：1min IHC image of CSB-RA249552A0HU diluted at 1:100 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA249552A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of NIH-3T3 cell with CSB-RA249552A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing 786-O cells stained with CSB-RA249552A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

P55884

Target Names

EIF3B

Alternative Names

eIF-3-eta; eIF3 eta; eIF3 p110; eIF3 p116; EIF3-ETA; EIF3-P110; EIF3-P116; eIF3b; EIF3B_HUMAN; EIF3S9; EIF3S9; formerly; Eukaryotic translation initiation factor 3; subunit B; Eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3 subunit 9 eta; Eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3; subunit 9 (eta; 116kD); eukaryotic translation initiation factor 3; subunit 9 eta; 116kDa; eukaryotic translation initiation factor 3; subunit 9; formerly; hPrt1; Protein synthesis defective at 36 degrees celsius 1; S. cerevisiae; homolog of; PRT1; Prt1 homolog

Species Reactivity

Human

Immunogen

A synthesized peptide from human EIF3B protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7E3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.; (Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.

Gene References into Functions

Study demonstrated that EIF3B expression was up-regulated in esophageal squamous cell carcinoma cells and negatively correlated with the prognosis of patients. PMID: 27270324
Novel RNA-binding protein P311 binds eIF3b to promote translation of TGF-beta1, TGF-beta2, TGF-beta3. PMID: 25336651
Report EIF3B binding within the IRES domain V of the coxsackie virus B3 mutant strain. PMID: 24063684
eIF3b expression relates to human bladder and prostate cancer prognosis, is required for tumor growth, and thus a candidate therapeutic target. PMID: 23575475
EIF3B promotes the proliferation of U87 human glioblastoma cells and may play an important role in human glioblastoma development. PMID: 22234522
Yeast eIF3b exhibits RNA binding activity but huamn eIF3b doesn't. PMID: 20862284
a conserved tryptophan residue in the human eIF3j N-terminal acidic motif (NTA) is held in the helix alpha1 and loop 5 hydrophobic pocket of the human eIF3b RNA recognition motif (RRM) PMID: 20060839
structural analysis of the eIF3b RNA recognition motif and its interaction with eIF3j PMID: 17190833

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Subcellular Location

Cytoplasm.

Protein Families

EIF-3 subunit B family

Database Links

HGNC: 3280

OMIM: 603917

KEGG: hsa:8662

STRING: 9606.ENSP00000354125

UniGene: Hs.371001

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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