GRIA2/GRIA3 Recombinant Monoclonal Antibody

GRIA2 and GRIA3 are core subunits of AMPA-type ionotropic glutamate receptors, the principal mediators of fast excitatory synaptic transmission in the central nervous system. These receptors play fundamental roles in synaptic plasticity, learning, and memory formation, making them essential targets for neuroscience research exploring everything from basic neuronal signaling to the molecular underpinnings of neurological disorders.

This recombinant monoclonal antibody, clone 4C3, offers researchers the reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures consistent performance across lots, eliminating the variability that can complicate long-term studies or multi-site collaborations. The antibody was raised in rabbit against a synthetic peptide derived from human GRIA2/GRIA3 and has been validated for human samples.

Validation studies confirm this antibody performs well in immunofluorescence applications, with recommended working dilutions of 1:20 to 1:200 providing flexibility to optimize signal intensity for your specific experimental conditions. Immunofluorescence staining of MCF-7 cells demonstrated clear detection when cells were fixed with formaldehyde, permeabilized with Triton X-100, and incubated with the antibody overnight at 4°C using a fluorescent secondary detection system. The antibody is also validated for ELISA, expanding its utility across different experimental workflows.

Supplied in a glycerol-containing buffer with BSA stabilizer, this affinity-purified antibody is ready for immediate use in studies investigating glutamatergic signaling, receptor trafficking, or synaptic function. Whether you are characterizing AMPA receptor expression patterns or exploring their roles in neuroplasticity, this antibody provides a dependable tool for your neuroscience research.