Product Details

Uniprot No.

Target Names

Alternative Names

EC 2.5.1.18 antibody; Glutathione S Transferase kappa 1 antibody; Glutathione S transferase subunit 13 antibody; Glutathione S-transferase k1 antibody; Glutathione S-transferase kappa 1 antibody; Glutathione S-transferase subunit 13 antibody; Glutathione S-transferase subunit 13 homolog antibody; GST 13 13 antibody; GST 13-13 antibody; GST antibody; GST class kappa antibody; GST class-kappa antibody; GST13 antibody; GST13-13 antibody; GSTK1 1 antibody; Gstk1 antibody; GSTK1-1 antibody; GSTK1_HUMAN antibody; hGSTK1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human GSTK1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

9E5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Glutathione S-transferase kappa 1 (GSTK1) belongs to a distinct class of glutathione transferases localized primarily to peroxisomes and mitochondria, where it plays essential roles in cellular detoxification and protection against oxidative stress. Unlike cytosolic GST family members, the kappa class enzymes are involved in lipid metabolism and the detoxification of reactive intermediates generated during fatty acid oxidation, making GSTK1 a valuable target for researchers investigating metabolic disorders, oxidative damage pathways, and peroxisomal biology.

This recombinant monoclonal antibody, generated from clone 9E5 in rabbit host, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, researchers benefit from lot-to-lot uniformity that eliminates the variability often encountered with traditional hybridoma-derived antibodies. This consistency is particularly valuable for longitudinal studies or when standardizing protocols across multiple experiments.

Validation testing demonstrates reliable performance across multiple applications. In western blot analysis, the antibody detects GSTK1 at the expected 25 kDa molecular weight across diverse human cell lines including Jurkat, SW620, HeLa, and PC-3, with optimal results achieved at dilutions between 1:500 and 1:2000. The concordance between predicted and observed band sizes confirms specific target recognition. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human stomach tissue shows clear staining at 1:50 to 1:200 dilutions, enabling researchers to examine GSTK1 expression patterns in archival specimens.

This antibody serves investigators exploring glutathione-dependent detoxification mechanisms, peroxisomal function, and the metabolic adaptations of cancer cells, providing a dependable tool for both protein expression analysis and tissue localization studies.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Significant glutathione conjugating activity is found only with the model substrate, 1-chloro-2,4-dinitrobenzene (CDNB).

Gene References into Functions

Several biological properties of the GST-hNdCTR1 fusion protein were assessed. It was demonstrated that in cells, the protein was prone to oligomerization, formed inclusion bodies and displayed no toxicity. Treatment of E. coli cells with copper and silver ions reduced cell viability in a dose- and time-dependent manner PMID: 29099786
we confirmed several existing chemoinformatic filters and more importantly extended them as well as added novel filters that specify compounds with anti-GST/GSH activity. Selected compounds were also tested using different antibody-based GST detection technologies and exhibited no interference clearly demonstrating specificity toward their GST/GSH interaction. PMID: 27044684
Our findings indicate that the medical staff exposed to low IR levels were under risk of significant oxidative stress that was enhanced by their glutathione S-transferase (GST) polymorphisms. PMID: 28287017
GSTK1 T/T genotype may be a novel risk factor for the prediction of overweight status in SCZ male patients. PMID: 27010189
High glutathione-S-transferase is associated with type 2 diabetes mellitus. PMID: 27377684
DsbA-L is localized in both the mitochondria and the endoplasmic reticulum (ER) in adipocytes; its ER localization plays a critical role in suppressing ER stress and promoting adiponectin biosynthesis and secretion. PMID: 25739441
we have optimized the GST-Nck1-SH2 pull-down procedure to obtain tyrosine-phosphorylated proteins in tumor tissues PMID: 23426619
drug resistance in three strains of tumor cells is associated with significant increase in hGSTP1 and hGSTA4 gene expression, whereas increased hGSTK1 gene expression was detected only in resistant erythroleukemia and mammary adenocarcinoma cells. PMID: 23330092
This study does not give evidence of interaction between the GST polymorphisms and smoking may although this study provided sufficient statistical power to detect modest interaction. PMID: 20472488
SNP-1308G/T (rs1917760) genotypes of DsbA-L gene might participate in insulin secretion and body fat distribution. It is possible that polymorphisms of DsbA-L gene associated with metabolic diseases[DsbA-L] PMID: 19225211
structure and function characterization of a GST from human breast PMID: 14709161
Gene and protein characterization; its subcellular localization in peroxisomes, suggesting a new function for this family of enzymes [glutathione S-transferase kappa (hGSTK1)] PMID: 14742434
crystal structure of hGSTK1 has been determined by the multiple-isomorphous replacement method and refined to 1.93 A resolution PMID: 16081649
Our results suggest that genetic polymorphisms of xenobiotic-metabolizing enzymes could play an important role in infertility. PMID: 18774560
The objective of this study was to investigate the molecular mechanisms underlying Group B Streptococcus-human umbilical vein endothelial cells interaction, focusing specifically on the responsiveness of host protein tyrosine kinase (PTK). PMID: 19639233
Observational study of gene-disease association. (HuGE Navigator) PMID: 19225211
Observational study of gene-disease association. (HuGE Navigator) PMID: 17601350
Presence of hGSTK1 in both peroxisomes and mitochondria. The C-terminus of hGSTK1 is essential for localization of the protein to peroxisomes, and the C-terminal sequence Ala-Arg-Leu represents a peroxisome targeting signal 1 (PTS1). PMID: 14742434

Hide All

Subcellular Location

Peroxisome.

Protein Families

GST superfamily, Kappa family

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 16906

OMIM: 602321

KEGG: hsa:373156

UniGene: Hs.390667

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA158046A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg),SW620 whole cell lysate(30µg), Hela whole cell lysate(30µg), PC-3 whole cell lysate(30µg) All lanes: GSTK1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time：3min IHC image of CSB-RA158046A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Have Questions?	Leave a Message or Start an on-line Chat

GSTK1 Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background