GSTM3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA009982MA1HU
Size	US$210
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Image	Western Blot Positive WB detected in: HeLa whole cell lysate(20µg), THP-1 whole cell lysate(20µg), PC-3 whole cell lysate(20µg), U-251MG whole cell lysate(20µg), Jurkat whole cell lysate(20µg), HepG2 whole cell lysate(20µg), A549 whole cell lysate(20µg) All lanes: GSTM3 antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time：2min Western Blot Positive WB detected in: Mouse kidney tissue lysate(20µg), Mouse liver tissue lysate(20µg), Rat skin tissue lysate(20µg) All lanes: GSTM3 antibody at 1:1000 Secondary Goat polyclonal to human IgG at 1/40000 dilution Predicted band size: 26.6 kDa Observed band size: 27 kDa Exposure time: 2min IHC image of CSB-RA009982MA1HU diluted at 1：100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA009982MA1HU diluted at 1：100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing MCF-7 cells stained with CSB-RA009982MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P21266

Target Names

GSTM3

Alternative Names

brain GST antibody; brain type mu glutathione S transferase antibody; glutathione S alkyltransferase M3 antibody; glutathione S aryltransferase M3 antibody; glutathione S transferase M3 (brain) antibody; glutathione S transferase M3 antibody; Glutathione S transferase Mu 3 antibody; glutathione S transferase, Mu 3 antibody; Glutathione S-transferase Mu 3 antibody; GST class mu 3 antibody; GST class-mu 3 antibody; GST5 antibody; GSTB antibody; Gstm3 antibody; GSTM3-3 antibody; GSTM3_HUMAN antibody; GTM3 antibody; hGSTM3-3 antibody; MGC3310 antibody; MGC3704 antibody; S (hydroxyalkyl)glutathione lyase M3 antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human GSTM3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

hIgG1

Clone No.

2H9D7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Glutathione S-transferase Mu 3 (GSTM3) plays a critical role in cellular detoxification pathways, catalyzing the conjugation of glutathione to a wide range of electrophilic compounds. Often referred to as brain-type GST due to its notable expression in neural tissues, GSTM3 has garnered significant research interest for its involvement in xenobiotic metabolism, oxidative stress responses, and potential associations with susceptibility to environmental toxins and disease states.

This recombinant monoclonal antibody, clone 2H9D7, offers researchers the consistency and reliability that comes with sequence-defined production. Unlike traditional hybridoma-derived antibodies, recombinant technology ensures lot-to-lot reproducibility, giving you confidence that your experimental results will remain comparable across studies and over time.

Validated across multiple applications, this antibody demonstrates genuine versatility for diverse experimental workflows. Western blot analysis confirms specific detection of GSTM3 at the expected 27 kDa molecular weight across an extensive panel of human cell lines including HeLa, THP-1, PC-3, U-251MG, Jurkat, HepG2, and A549. Cross-species reactivity extends to mouse and rat samples, with successful detection demonstrated in mouse kidney and liver tissue lysates as well as rat skin tissue. For spatial expression studies, immunohistochemistry validation in human testis and kidney tissues shows clear staining patterns using citrate buffer antigen retrieval. Flow cytometry applications have been confirmed in MCF-7 cells, enabling quantitative analysis of intracellular GSTM3 levels.

This antibody serves researchers investigating phase II detoxification enzymes, drug metabolism pathways, oxidative stress mechanisms, and toxicological responses across multiple model systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.

Gene References into Functions

Differential activity of antioxidant enzymes caused by the polymorphism in GSTM3 may contribute to resistance to hormonal therapy through oxidative stress. The GSTM3 rs7483 polymorphism may be a promising biomarker for prostate cancer patients treated with androgen-deprivation therapy (ADT) PMID: 27993795
Expression of GSTM3 might be regulated by epigenetic changes in lens tissue. Hypermethylation in GSTM3 promoter and altered histone modification might have a role in the ARC formation. PMID: 27607418
No associations between the GSTT1, GSTP1, and GSTM3 genotypes and neoplasia risk were observed. In conclusion, we determined the genotype distribution of GST polymorphisms in control subjects and breast cancer patients from northeastern Mexico. PMID: 26125851
NSD1 interacted with RNAPII and bound to GSTM3 -63A/C TATA box. PMID: 25193115
All three markers correlated significantly with regional lymph node metastasis: FXYD3 (p = 0.0110), S100A11 (p = 0.0071), and GSTM3 (p = 0.0173) in colon cancer lymphatic metastasis. PMID: 22430872
This meta-analysis suggests that the GSTM3 A/B polymorphism may be an important protective factor for head and neck cancer, especially of laryngeal cancer and Caucasian populations. PMID: 24416175
rs1332018 genetic variants in the GSTM3 promoter predispose the host to downregulating GSTM3 expression in kidney, facilitate carcinogenesis, and predict an unfavourable postoperative prognosis of renal cell carcinoma. PMID: 24157827
In conclusion, this epistatic interaction showed a high degree of consistency when stratifying by sex, the epsilon4 allele of apolipoprotein E genotype, and geographic region. PMID: 23036584
The GSTM3 A/B gene polymorphism is not associated with lung cancer susceptibility. PMID: 23167362
Methylation of GSTM3 promoter may contribute to oxidative stress-associated liver damage and correlate with the disease severity in Acute-on-chronic hepatitis B liver failure. PMID: 22976281
There were no significant differences in distribution of 3-bp deletion polymorphism in intron 6 variant allele in Glutathione-S-transferase M3 between chronic obstructive pulmonary disease patients and controls in a north Indian population. PMID: 21513434
In individuals from Angola, Mozambique and the Sao Tome e Principe islands, the GSTM3*B allele was three times more frequent (0.74-0.78) than the GSTM3*A allele (0.22-0.26), with no significant differences in allele frequency across the three groups. PMID: 20549140
Diminished GSTM3 mRNA levels correlated with decreased minichromosome maintenance deficient 3 (MCM3) mRNA levels in a diagnostic and SNP-dependent fashion in Alzheimer disease. PMID: 18423940
The presence of the GSTM3*B allele appears to associated with a reduced risk of laryngeal squamous cell carcinoma. PMID: 19922706
No increased prostate scancer risk for men carrying any of the GSTM1 or GSTT1 genotypes. PMID: 14968442
Glutathione S-transferase hGSTM3 has a role in ageing-associated neurodegeneration PMID: 15621212
GSTM3 -63C allele strongly affects gene expression and suggests that individuals who carry the low expression allele may be deficient in glutathione transferase catalyzed biological functions. PMID: 15665284
Single marker association analyses revealed that the AGG/AGG genotype of the GSTM3 rs1799735 (del/AGG) polymorphism was associated with an increased risk of AD (p=0.05), especially in the group of APOE4-allele non-carriers (p=0.004; OR=2.07). PMID: 17904251
distribution of -63A/C polymorphism of human glutathione S-transferase M3(GSTM3) gene in Chinese Han population and the association of -63A/C polymorphism with essential hypertension PMID: 17922434
The GSTM3*A/*A genotype was 76% in cancer cases versus 74% in controls; a significant association between smoking status and GSTM3 genotype was not detected. PMID: 18569590
Our results suggested GSTM3 (AB + BB) genotype to be significantly associated with prostate cancer risk. PMID: 18668224
GST-M3 activity is possibly involved in protection against mucosal atrophy caused by H. pylori as the levels of IgG titer and pepsinogen I are linked to mucosal status. PMID: 19696791
The researchers found evidence of an increased risk for non-Hodgkin's lymphoma in women who used hair dye before 1980 and who were carriers of the GSTM3 intron 6 deletion. PMID: 19822571

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Subcellular Location

Cytoplasm.

Protein Families

GST superfamily, Mu family

Tissue Specificity

Testis and brain.

Database Links

HGNC: 4635

OMIM: 138390

KEGG: hsa:2947

STRING: 9606.ENSP00000256594

UniGene: Hs.2006

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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