Product Details

Uniprot No.

Q00613

Target Names

HSF1

Alternative Names

Heat shock factor protein 1 (HSF 1) (Heat shock transcription factor 1) (HSTF 1), HSF1, HSTF1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human HSF1

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

5C7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Heat shock factor protein 1 (HSF1) serves as the master regulator of the cellular heat shock response, orchestrating the transcription of heat shock proteins that protect cells from proteotoxic stress. Beyond its classical role in stress adaptation, HSF1 has emerged as a critical factor in cancer biology, where it supports the enhanced protein folding demands of rapidly proliferating tumor cells and influences metabolic reprogramming. This positions HSF1 as a compelling target for researchers investigating stress signaling pathways, tumor microenvironment dynamics, and potential therapeutic vulnerabilities in malignancies.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human HSF1, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. As a recombinant clone (5C7), it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring that your optimized protocols remain stable across extended studies and collaborative projects.

The antibody has been validated for immunohistochemistry applications, with demonstrated performance in paraffin-embedded human tissues. Testing in human ovarian cancer tissue and human testis tissue at 1:100 dilution using citrate buffer antigen retrieval confirms reliable detection in both neoplastic and normal tissue contexts. The recommended working range of 1:50–1:200 provides flexibility for optimization across different tissue types and detection systems.

Supplied in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C, this antibody supports research programs spanning epigenetics, nuclear signaling, and cardiovascular biology. For investigators exploring HSF1's expanding roles in stress adaptation and cancer progression, this recombinant tool delivers the consistency essential for meaningful, reproducible results.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Functions as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage. In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form. Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes. Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form. Binds to inverted 5'-NGAAN-3' pentamer DNA sequences. Binds to chromatin at heat shock gene promoters. Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells. Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner. Plays a role in nuclear export of stress-induced HSP70 mRNA. Plays a role in the regulation of mitotic progression. Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner. Involved in stress-induced cancer cell proliferation in a IER5-dependent manner.; (Microbial infection) Plays a role in latent human immunodeficiency virus (HIV-1) transcriptional reactivation. Binds to the HIV-1 long terminal repeat promoter (LTR) to reactivate viral transcription by recruiting cellular transcriptional elongation factors, such as CDK9, CCNT1 and EP300.

Gene References into Functions

HSF1 may be closely associated with the proliferation and motility of gastric cancer cells and poor prognosis of patients with gastric cancer. Accordingly, HSF1 could serve as a prognostic marker for gastric cancer. PMID: 30328318
HSF1 positively regulates the transcription of latent HIV PMID: 27189267
A strong reduction in heat shock transcription factor 1 (HSF1) levels was evident in Huntington's Disease (HD). PMID: 28194040
Variations in brain defects result from cellular mosaicism in the activation of Hsf1 heat shock signaling. PMID: 28462912
Studies indicate that heat shock factor 1 (HSF1) acts in diverse stress-induced cellular processes and molecular mechanisms. PMID: 29774376
In response to DNA damage, activated and auto-poly-ADP-ribosylated PARP1 dissociates from HSF1-PARP13, and redistributes to DNA lesions and DNA damage-inducible gene loci. PMID: 29158484
Overexpressed HSF1 triggers pre-mRNA 3' processing in cancers. PMID: 29268782
High HSF1 expression in tumor tissues may be a prognostic biomarker in patients with intrahepatic cholangiocarcinoma. PMID: 29278438
Studies suggest that heat shock factor 1 (HSF1) serves to integrate diverse biological and pathological responses [Review]. PMID: 28890254
The single nucleotide polymorphism rs78202224 (G>T) was significantly associated with increased risk of breast cancer. PMID: 29494616
results thus contribute to the knowledge of the regulatory mechanism of HSF1 in down-regulating ArgBP2, providing new insight into the HSF1&MORC2-PRC2-ArgBP2 signaling pathway and a better understanding of their functions in gastric cancer cells. PMID: 29339121
These findings indicate that activation of HSF1 at Ser326 residue and transcription of HSP27 is related to the maintenance of gynecological CSCs/CICs. PMID: 28415561
Results show that HSF1 is a key transcription factor for inducing the expression of DNAJB8 and SOX2 and that cellular stress induces cancer stem-like cells through the expression of DNAJB8 by activation of HSF1 . PMID: 29316077
The mRNA expression levels of heat shock transcription factor 1 (HSF1) in estrogen receptor (ER)-positive breast cancer are associated with both shorter relapse-free and overall survival. PMID: 27713164
Our study provides evidence that HSF1 functions as a novel oncogene in pancreatic tumors and is implicated as a target for the diagnosis and treatment of pancreatic cancer. PMID: 28482903
In normal ovarian tissues, HSF1 was barely detected, whereas, high expression of HSF1 was found in malignant epithelial ovarian cancer (EOC) tissues, including serous, mucinous, endometrioid, and clear cell EOC tissues. PMID: 28487934
Our findings show that miR-487a, mediated by heat shock factor 1, promotes proliferation and metastasis of Hepatocellular carcinoma (HCC) by PIK3R1 and SPRED2 binding, respectively. Our study provides a rationale for developing miR-487a as a potential prognostic marker or a potential therapeutic target against HCC. PMID: 27827315
HSF1 activity is decreased in fibrotic hearts. HSF1 inhibits phosphorylation and nuclear distribution of Smad3 via direct binding to Smad3. Active Smad3 blocks the anti-fibrotic effect of HSF1. PMID: 28091697
Results suggest for targeting heat shock factor 1 (HSF1)activation in combination with bortezomib to enhance multiple myeloma treatment efficacy. PMID: 27487129
MD simulation of high-resolution X-ray structures reveals post-translational modification dependent conformational changes in HSF-DNA interaction. PMID: 27882499
We found that HSF1 activation mediated by 1,4-NQ upregulated downstream genes, such as HSPA6. The results suggest that activation of the HSP90-HSF1 signal transduction pathway mediated by 1,4-NQ protects cells against 1,4-NQ and that per/polysulfides can diminish the reactivity of 1,4-NQ by forming sulfur adducts. PMID: 28049024
casein kinase 1 phosphorylates the SQSTM1 S349 residue when harmful proteins accumulate under HSF1 stress PMID: 27846364
Evidence for the essential function of HSF1 in the transcriptional activation of TERRA and in telomere protection upon stress. PMID: 28369628
Acetylation of the protein triggers TDP-43 pathology in cultured cells and mouse skeletal muscle, which can be cleared through an HSF1-dependent chaperone mechanism that disaggregates the protein. PMID: 28724966
Low glucose culture hampered typical epithelial-mesenchymal transition-like morphological change, "cadherin switching," and cell migration of hepatocellular carcinoma cells through inducing persistent down-regulation of HSF1, resulting in direct inhibition of snail1 expression. PMID: 27755964
piR-823 increased the transcriptional activity of HSF1, the common transcription factor of HSPs, by binding to HSF1 and promoting its phosphorylation at Ser326. PMID: 28618124
Reporter assay showed that HSF1 increased the transcriptional activity of ATG4B gene promoter, and chromatin immunoprecipitation assay verified that HSF1 bound to the site (-1429 to -1417) in ATG4B gene promoter region. PMID: 28889000
Knockdown of HSF1 reduced the proliferation, migration and invasion of osteosarcoma cells, while overexpression of HSF1 promoted the proliferation, migration and invasion of osteosarcoma cells. PMID: 28370690
Studies indicate correlations between heat shock transcription factor 1 (HSF1) activity and the incidence of several cancer types. PMID: 27225066
These findings suggest that HSF1 is important in the ovarian cancer TGFbeta response and in Epithelial-Mesenchymal Transition. PMID: 27997575
BRD4 regulates splicing during heat shock by interacting with HSF1 such that under heat stress BRD4 is recruited to nuclear stress bodies, and non-coding SatIII RNA transcripts are up-regulated. PMID: 27536004
Results demonstrate that p38 MAPK not only causes phosphorylation of HSF1 at S326 but also at S303/307 , and transcriptionally activates HSF1. PMID: 27354066
HSF1 translationally augments the proteotoxic stress response. PMID: 27043084
Heat Shock Factor 1 Depletion Sensitizes A172 Glioblastoma Cells to Temozolomide via Suppression of Cancer Stem Cell-Like Properties. PMID: 28241425
By showing transcription factor HSF1 activation, we demonstrated that HCA induces the expression of BAG3 through HSF1 activation. More importantly, knockdown of BAG3 expression using siRNA largely inhibited HCA-induced apoptosis, suggesting that BAG3 is actively involved in HCA-induced cancer cell death PMID: 27922674
miR-34b suppressed AML cell proliferation and survival by targeting HSF1, in turn leading to the inactivation of Wnt-beta-catenin pathway, which may highlight a new therapeutic approach for AML. PMID: 27296951
The evidence of genetic associations has been found for the multivariate response phenotype that involves trans effects modulating expression of genes following heat shock, including HSF1 and UBQLN1. PMID: 27553423
M3-mAChR activation leads to enhancement of hsp expression via PKC-dependent phosphorylation of HSF1, thereby stabilizing the mutant hERG-FLAG protein. Thus, M3-mAChR activators may have a therapeutic value for patients with LQT2. PMID: 27803431
Oncogenic signaling mobilizes HSF1, cancer cells rely on HSF1 to avert proteomic instability and repress tumor-suppressive amyloidogenesis. [review] PMID: 26597576
These results reveal the existence of a novel IER5-mediated cancer regulation pathway that is responsible for the activation of HSF1 observed in various cancers. PMID: 26754925
The authors found a temperature-dependent unfolding of Hsf1 in the regulatory region happening concomitant to tighter packing in the trimerization region. PMID: 26785146
Ginsenoside Rg3 induces FUT4-mediated apoptosis in H. pylori CagA-treated gastric cancer cells by regulating SP1 and HSF1 expressions PMID: 26427350
Aberrant HSF1 degradation is a key neurodegenerative mechanism underlying alpha-synucleinopathy. Elevated NEDD4 is implicated as the responsible ubiquitin E3 ligase for HSF1 degradation through ubiquitin-proteasome system. PMID: 26503960
High HSF1 expression is associated with acute myeloid leukemia. PMID: 26473447
Glucose, but not 2D-glucose, can induce the phosphorylation of HSF1 at S326 and upregulate the expression of HSF1's downstream alpha B-crystallin and Hsp70 PMID: 26010766
Data show that tonantzitlolone (TZL) was able to induce protein kinase c theta (PKCtheta;)-dependent heat shock transcription factor 1 (HSF1)phosphorylation. PMID: 26298773
Data suggest that heat shock factor 1 (HSF1) interacts with both Ku autoantigens Ku70 and Ku86 to induce defective non-homologous end joining (NHEJ) repair activity and genomic instability. PMID: 26359349
Suggest that the early activation of Hsf1 dependent cell stress pathway by mono-allelic mutations in APC can affect cell programming in a way that contributes to cancer onset. PMID: 26320184
The study presents cocrystal structures of the human HSF1 DNA-binding domain in complex with cognate DNA. PMID: 26727489
Phosphorylation of HSF1 at Ser230 is responsible for Hsp70-1 upregulation during coxsackieviral infection. PMID: 26361762

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Subcellular Location

Nucleus. Cytoplasm. Nucleus, nucleoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore.

Protein Families

HSF family

Database Links

HGNC: 5224

OMIM: 140580

KEGG: hsa:3297

STRING: 9606.ENSP00000431512

UniGene: Hs.530227

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Code	CSB-RA279005A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA279005A0HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA279005A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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HSF1 Recombinant Monoclonal Antibody

Product Details

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Target Background