Code | CSB-RA279005A0HU |
Size | US$210 |
Order now | |
Image |
|
Have Questions? | Leave a Message or Start an on-line Chat |
Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
To produce the HSF1 recombinant monoclonal antibody, several intricate and sequential steps are required. The first step involves harvesting the HSF1 monoclonal antibody and sequencing its gene. Next, a vector containing the HSF1 monoclonal antibody gene is constructed and transfected into a host cell line for culturing. The HSF1 monoclonal antibody is then produced by synthesizing a peptide derived from human HSF1 as an immunogen. Afterward, the HSF1 recombinant monoclonal antibody is purified through affinity chromatography to ensure high purity. Finally, the specificity of the antibody is confirmed through ELISA and IHC assays, which test its capacity to identify HSF1 accurately. It only detects HSF1 human protein.
The HSF1 protein is a transcription factor that plays a critical role in the cellular response to stress by regulating the expression of genes involved in the heat shock response and proteostasis. HSF1 regulates the expression of genes that encode heat shock proteins (HSPs) in response to elevated temperatures. HSPs are molecular chaperones that help protect cells from the deleterious effects of stress by facilitating protein folding and preventing protein aggregation. HSF1 promotes the expression of genes involved in protein folding, degradation, and autophagy, thereby maintaining proteostasis under stress conditions. It is also involved in the regulation of cell growth and survival. Dysregulation of HSF1 has been associated with various diseases, including cancer, neurodegenerative diseases, and cardiovascular diseases.
There are currently no reviews for this product.