HSPA14 Recombinant Monoclonal Antibody

HSPA14, also known as heat shock 70 kDa protein 14 or HSP70-like protein 1, belongs to the HSP70 family of molecular chaperones that play essential roles in protein folding, cellular stress responses, and proteostasis maintenance. This protein participates in the cellular machinery that protects against misfolded protein accumulation, making it a valuable target for researchers investigating stress response pathways, protein quality control mechanisms, and related disease processes.

This recombinant monoclonal antibody, clone 4G5, offers the consistency and reliability that demanding research applications require. Produced using recombinant technology with a defined sequence, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format, raised against a synthesized peptide from human HSPA14, has been affinity-purified to ensure high specificity for your target.

Validation studies demonstrate robust performance across multiple experimental platforms. Immunohistochemistry testing in paraffin-embedded human tissues reveals clear cytoplasmic staining patterns in both colorectal cancer and testis samples at 1:100 dilution using citrate buffer antigen retrieval. For flow cytometry applications, the antibody successfully detects HSPA14 in Raji cells following formaldehyde fixation and Triton X-100 permeabilization, showing distinct positive signal separation from isotype control. These validated applications at dilutions ranging from 1:50 to 1:200 provide flexibility for optimization in your specific experimental conditions.

Whether you are exploring chaperone biology in cancer contexts, investigating stress response mechanisms, or examining protein homeostasis in reproductive tissues, this antibody provides a reliable tool for advancing your research into HSP70 family function and regulation.