Code | CSB-RA802195A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
B cells were extracted from an animal immunized with a synthesized peptide derived from human HTT, which was then fused with myeloma cells to form hybridomas. The variable light (VL) and variable heavy (VH) domains of HTT antibody-producing hybridomas were sequenced, and this sequencing data was used for vector construction in the recombinant generation. The HTT monoclonal antibody gene-containing vector was then transfected into cells for culture. The HTT recombinant monoclonal antibody was purified from the cell culture supernatant using affinity chromatography. The purified antibody was found to exhibit specificity in ELISA and IHC applications, detecting only the human HTT protein.
The HTT protein is involved in various cellular processes, including transcriptional regulation, vesicle trafficking, and cell signaling. It is primarily expressed in the brain, where it plays an important role in neuronal development and function. The mutation of HTT protein is associated with Huntington's disease, which is believed to interfere with normal cellular processes, leading to the death of brain cells and the characteristic symptoms of the disease.
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