ITGAL Antibody

Code CSB-RA787996A0HU
Size US$350 How to order?
Image
  • Western Blot
    Positive WB detected in: THP-1 whole cell lysate
    All lanes: ITGAL antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 129, 135, 120 kDa
    Observed band size: 130 kDa
  • Immunofluorescence staining of THP-1 cell with CSB-RA787996A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 490-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details

Uniprot No. P20701
Target Names ITGAL
Alternative Names Integrin alpha-L (CD11 antigen-like family member A) (Leukocyte adhesion glycoprotein LFA-1 alpha chain) (LFA-1A) (Leukocyte function-associated molecule 1 alpha chain) (CD antigen CD11a), ITGAL, CD11A
Species Reactivity Human
Immunogen A synthesized peptide derived from human ITGAL
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Clone No. 2B2
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IF Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. Integrin ITGAL/ITGB2 is a receptor for F11R. Integin ITGAL/ITGB2 is a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL. Involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Contributes to natural killer cell cytotoxicity. Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils. Required for generation of common lymphoid progenitor cells in bone marrow, indicating a role in lymphopoiesis. Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
Gene References into Functions
  1. LFA-1 cross-linking recruits and activates FAK1 and PYK2 to phosphorylate LAT selectively on a single Y-171 site that binds to the GRB2-SKAP1 complex and limits dwell times of T-cells with dendritic cells PMID: 28699640
  2. Data show that integrin alphaLbeta2 (LFA-1) is oriented relative to actin retrograde flow. PMID: 29229906
  3. These results demonstrated that histone demethylase JMJD3 regulates CD11a expression in lupus T cells by affecting the H3K27me3 levels in the ITGAL (CD11a) promoter region, and JMJD3 might thereby serve as a potential therapeutic target for SLE. PMID: 28430662
  4. Data show a nadir of CD11a expression at 2 years of natalizumab treatment in multiple sclerosis, at the peak of incidence of progressive multifocal leukoencephalopathy, indirectly suggesting that a lack of these molecules may play a role in the onset of PML in NTZ-treated patients. PMID: 24258149
  5. Extracellular ISG15 signals cytokine secretion through the LFA-1 integrin receptor (CD11a/CD18) in natural killer cells. PMID: 29100055
  6. Suggest possible role for lymphocyte CD11a in the pathogenesis immune thrombocytopenia. PMID: 26884833
  7. The alphaI domain, tethered through its N-linker and a disulfide to a stable beta-ribbon pillar near the center of the platform, can undergo pivoting and tilting motions that appear buffered by N-glycan decorations that differ between alphaL and alphaX subunits. PMID: 26936951
  8. beta2 integrins-mediated chemosensory adhesion and migration of polymorphonuclear leukocytes on the vascular graft surface, bacterial cellulose. PMID: 25231265
  9. Data show that interleukin-18 was involved in the eosinophil-mediated death of colon carcinoma cells via upregulation of CD11a/CD18 and ICAM-1 adhesion molecules. PMID: 26216891
  10. shear-induced CD11/CD18 cleavage is phenotypic of neutrophilic cells, including those derived from HL60 cells PMID: 24276331
  11. Data show that leucocyte function-associated antigen-1 (integrin alphaLbeta2), DNAX accessory molecule-1 (DNAM-1) or tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) enhance osteoclast survival when co-cultured with activated NK cells. PMID: 25684021
  12. LFA-1 function is regulated through conformational changes that include the modulation of ligand binding affinity and molecular extension. PMID: 25378583
  13. the blocking of LFA-1/ICAM-1 interaction resulted in a significant increase in the percentage of IL-17(+) T cells expanded in nonclassical monocyte/T-cell cocultures PMID: 25678252
  14. Studied signaling mechanisms of Lymphocyte function-associated antigen-1 (LFA-1) using NMR analysis of the dynamics of the LFA-1 I-domain. PMID: 25147050
  15. CD11a, CD11c, and CD18 gene polymorphisms and susceptibility to Behcet's disease in Koreans. PMID: 25155097
  16. ITGAL is overexpressed in systemic lupus erythematosus CD4+ T cells, but expression is not linked to the typical clinical and serological parameters associated with the disease. PMID: 24238281
  17. there was reduced expression of CD11a, CD11b, and CD18 in trisomy 12 cases with NOTCH1 mutations compared with wild type. PMID: 24829201
  18. We conclude, that deficiency of CD11a expression should be added to the diagnostic criteria of AML-M7, classical DS-AML and TMD. PMID: 23450818
  19. genetic polymorphism is associated with atopic disease and relevant allergic indices PMID: 23726040
  20. Distribution of integrin beta(2) is also markedly altered in M. tuberculosis-infected DCs. A corresponding reduction in the alphaL (CD11a) and alphaM (CD11b) subunits that associate with integrin beta(2) was also observed. PMID: 23981064
  21. Allele frequencies were determined in the blood donor population as follows: 0.318 for HNA-1a, 0.668 for HNA-1b, 0.014 for HNA-1c, 0.768 for HNA-3a, 0.232 for HNA-3b, 0.882 for HNA-4a, 0.118 for HNA-4b, 0.736 for HNA-5a and 0.264 for HNA-5b. PMID: 23398146
  22. The results of this study suggest that polyamine-induced suppression of LFA-1 expression occurs via enhanced methylation of ITGAL. PMID: 23418509
  23. Binding kinetics differences between LFA-1 and macrophage-1 antigen (Mac-1) are quantified and insight provided into their distinct functions in the neutrophil inflammatory cascade. PMID: 23514737
  24. common and rare variants in FTO, SERPINA12, and ITGAL predict PON1 acti PMID: 23160181
  25. Flow-based adhesion assay showed that engagement of CD103 or LFA-1, together with TCR, enhances the strength of the T-cell/target cell interaction PMID: 23188505
  26. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A PMID: 22384114
  27. Binding of LtxA to LFA-1 resulted in the internalization of both LtxA and LFA-1, with LtxA localizing specifically to the lysosomal compartment. PMID: 22467872
  28. This work demonstrates an intricate coupling between conformation and lateral diffusion of LFA-1 and further underscores the crucial role of mobility for the onset of LFA-1 mediated leukocyte adhesion. PMID: 22411821
  29. DNA hypermethylation at the CD11a locus contributes to the lowered expression of CD11a in biliary atresia CD4+ T cells. PMID: 22206678
  30. CD70 and CD11a facilitate the survival of T and B lymphocytes and indirectly enhance the destruction of platelets in immune thrombocytopenia. PMID: 21541792
  31. Regulator of G protein signaling (RGS) represses Galpha-dependent signaling during chemokine-activated integrin alphaLbeta2- and alpha4beta1- dependent T cell adhesion. PMID: 21705617
  32. N-terminal myr-tagged SKAP1 for membrane binding facilitated constitutive RapL membrane and Rap1 binding and effectively substituted for PI3K and TCR ligation in the activation of LFA-1 in T cells. PMID: 21669874
  33. hyperketonemia can induce monocyte adhesion to endothelial cells and it is mediated via increased ICAM-1 expression in endothelial cells and increased expression and affinity of LFA-1 in monocytes PMID: 21540444
  34. We show that sequential cleavage of C-terminal amino acids from the beta(2) cytoplasmic tail of LFA-1, by cathepsin X, enhances binding of the adaptor protein talin to LFA-1 and triggers formation of the latter's high-affinity form PMID: 21454358
  35. Bordetella pertussis adenylate cyclase toxin binds to T cells via LFA-1 and induces its disengagement from the immune synapse PMID: 21576384
  36. Studies indicate that transformation of LFA1 from a bent, resting form to an extended conformation is essential for T cell migration and the formation of an immunological synapse. PMID: 21597477
  37. LFA-1-induced stabilization of ARE-containing mRNAs in T cells is dependent on HuR, and occurs through the Vav-1, Rac1/2, MKK3 and p38MAPK signaling cascade PMID: 21206905
  38. RFX1 recruits SUV39H1 to the promoter regions of the CD11a and CD70 genes in CD4(+) T cells, thereby regulating local H3K9 tri-methylation levels. PMID: 21192791
  39. 2B4-mediated increase in natural killer (NK) cell adhesion to tumor targets is actin- and LFA-1-dependent, demonstrating that inside-out signaling is very fast and its effects can be detected as early as 90 seconds after cell contact. PMID: 21270395
  40. Findings support the idea that calpain is involved in the turnover of LFA-1 adhesions at the rear of the cell. PMID: 21152086
  41. the high-affinity state of LFA-1 on T cells is critical to induce targeted movements of both ICAM-1 and MHC class II to the immunological synapse on APCs. PMID: 20681010
  42. Decreased chemotactic response and downregulated expression of CD11a in an experimental model of depression could be an important mechanisms to induce impaired immune responses in experimental and clinical depression. PMID: 20516718
  43. Case Report: Possible acceleration of regression by antibiotic treatment in benign cephalic histiocytosis with infiltration of CD11a/CD11c+ macrophages. PMID: 20574620
  44. Human neutrophil rolling on E- or P-selectin induces the extended alpha(L)beta(2) integrin conformation through signaling triggered by PSGL-1 engagement. PMID: 20445017
  45. T helper (Th)17 cells adhere to mesenchymal stem cells through the joined involvement of CCR6 and CD11a/CD18 and their respective ligands, interactions that induce a regulatory phenotype in fully polarized tissue-infiltrating Th17 cells. PMID: 20511548
  46. These results provide an example of the stabilizing effect of polar interactions within the low dielectric environment of the membrane interior and demonstrate its importance in the regulation of alpha L beta 2 function. PMID: 20338181
  47. Activation-induced conformational changes in the I domain region of lymphocyte function-associated antigen 1 PMID: 11792712
  48. important role for the integrin LFA-1 and the microtubular cytoskeleton in the Ca(2)(+)-mediated activation of PYK2 in T-lymphocytes PMID: 11867690
  49. regulation of LFA-1 activation and neutrophil arrest on intercellular adhesion molecule 1 in shear flow PMID: 11929876
  50. cd11a may play an important role in pathogenic process(in COPD) PMID: 11953106

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Subcellular Location Cell membrane; Single-pass type I membrane protein.
Protein Families Integrin alpha chain family
Tissue Specificity Leukocytes.
Database Links

HGNC: 6148

OMIM: 153370

KEGG: hsa:3683

STRING: 9606.ENSP00000349252

UniGene: Hs.174103

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