Code | CSB-RA222329A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
The KDM1A recombinant monoclonal antibody is generated using protein technology and DNA recombinant technology. First, mice are immunized with a synthesized peptide derived from human KDM1A. Next, the spleen of mice is removed under sterile conditions, and total RNA is extracted from spleen cells. The cDNA, synthesized by RNA reverse transcription, serves as the template for PCR amplification of the KDM1A antibody gene. The resulting KDM1A antibody gene is then introduced into a vector and transfected into host cells for culturing. Finally, the KDM1A recombinant monoclonal antibody is purified from the supernatant of cell culture using affinity chromatography. It is thoroughly validated and can be used for the detection of human KDM1A protein in ELISA, WB, and IHC experiments.
KDM1A, also known as LSD1, is a histone demethylase that plays a crucial role in regulating gene expression by removing methyl groups from lysine 4 of histone H3 (H3K4) and interacting with transcription factors and co-regulators. KDM1A can interact with the androgen receptor (AR) and estrogen receptor (ER) to modulate the transcriptional activity of their target genes. It is also involved in other cellular processes, including DNA damage repair, cell proliferation, and differentiation.
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