LGR6 Recombinant Monoclonal Antibody

Code CSB-RA156336A0HU
Size US$210
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Image
  • IHC image of CSB-RA156336A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.26% DAB.
  • Immunofluorescence staining of hela with CSB-RA156336A0HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 500-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
  • Overlay Peak curve showing Hela cells surface stained with CSB-RA156336A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details

Uniprot No.
Target Names
LGR6
Alternative Names
Leucine-rich repeat-containing G-protein coupled receptor 6, LGR6
Species Reactivity
Human
Immunogen
A synthesized peptide derived from Human LGR6
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone No.
15G3
Purification Method
Affinity-chromatography
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Liquid
Tested Applications
ELISA, IHC, IF, FC
Recommended Dilution
Application Recommended Dilution
IHC 1:50-1:200
IF 1:50-1:200
FC 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The LGR6 recombinant monoclonal antibody is synthetically generated in vitro, employing a multi-step process. Initially, LGR6 antibody genes are isolated from B cells sourced from immunoreactive rabbits. These LGR6 antibody genes are then amplified and cloned into phage vectors, which are subsequently introduced into mammalian cell lines to facilitate the production of functional antibodies in significant quantities. The resulting LGR6 recombinant monoclonal antibody is purified from the culture supernatant of these transfected cell lines through affinity chromatography. It is well-suited for various applications, including ELISA, IHC, IF, and FC, enabling the precise detection of human LGR6 protein.

LGR6 is a cell-surface receptor protein involved in the regulation of tissue development, regeneration, and stem cell maintenance in various tissues, including the skin, hair follicles, and mammary glands. Its role in maintaining stem cell populations and regulating tissue homeostasis is crucial for normal tissue function and repair.

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Target Background

Function
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. May act as a tumor suppressor.
Gene References into Functions
  1. Inhibition of LGR6 promoted the osteogenic differentiation of bone marrow stromal cells in vitro. PMID: 29625528
  2. Lgr6 has a tumorpromoting role in the development of colorectal cancer. PMID: 29693156
  3. Lgr6 marks mammary gland progenitor cells that can initiate tumours, and cells of luminal breast tumours required for efficient tumour maintenance. PMID: 27798604
  4. Two of the three monoclonal antibodies recognized the large N-terminal extracellular domain of leucine rich repeat containing G protein-coupled receptor 6 (LGR6), and competitively blocked the binding of R-spondin 1, which is known to be the ligand for LGR6. PMID: 28013222
  5. LGR6 expression is significantly downregulated in human masticatory mucosa during wound healing PMID: 28005267
  6. Overexpression of wild-type LGR6 in HeLa cells leads to increased cell migration. PMID: 22615920

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Database Links

HGNC: 19719

OMIM: 606653

KEGG: hsa:59352

STRING: 9606.ENSP00000356247

UniGene: Hs.497402

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