LGR6 Recombinant Monoclonal Antibody

LGR6 (Leucine-rich repeat-containing G-protein coupled receptor 6) belongs to a family of receptors that play critical roles in stem cell biology and tissue homeostasis. As a marker of distinct stem cell populations in various tissues, LGR6 has emerged as an important target for researchers investigating regenerative processes, developmental biology, and cancer stem cell dynamics within signal transduction pathways.

This recombinant monoclonal antibody, clone 15G3, offers the reproducibility and consistency that demanding research applications require. Developed using recombinant technology with a sequence-defined approach, this antibody eliminates the lot-to-lot variability that can compromise longitudinal studies and multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide derived from human LGR6, provides reliable specificity for your experimental workflows.

Validation studies demonstrate robust performance across multiple detection platforms. In immunohistochemistry, the antibody successfully identifies LGR6 in paraffin-embedded human lung cancer tissue using standard citrate buffer antigen retrieval, providing clear visualization with DAB chromogen detection. Immunofluorescence studies in HeLa cells reveal distinct staining patterns when counterstained with DAPI, enabling subcellular localization analysis. Flow cytometry validation confirms the antibody's utility for surface staining applications, with clear population separation demonstrated in HeLa cells compared to isotype controls.

The antibody arrives affinity-purified in a glycerol-containing buffer optimized for long-term stability at -20°C or -80°C. With validated applications spanning ELISA, IHC, IF, and flow cytometry, this antibody supports diverse experimental approaches for researchers exploring LGR6 biology in stem cell research, tissue regeneration studies, and oncology investigations where understanding receptor-mediated signaling is essential.