LIG1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA877322A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate All lanes: DNA Ligase I antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 102, 89, 99 kDa Observed band size: 140 kDa IHC image of CSB-RA877322A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA877322A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Uniprot No.

P18858

Target Names

LIG1

Alternative Names

DNA ligase 1 (EC 6.5.1.1) (DNA ligase I) (Polydeoxyribonucleotide synthase [ATP] 1), LIG1

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human DNA Ligase I

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7B2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

DNA Ligase I plays an essential role in DNA replication and repair, catalyzing the final phosphodiester bond formation that joins Okazaki fragments during lagging strand synthesis and seals nicks generated during base excision repair. As a critical enzyme in maintaining genomic integrity, LIG1 has become an important target for researchers investigating DNA damage responses, cell cycle regulation, and the molecular mechanisms underlying cancer development.

This recombinant monoclonal antibody, generated from clone 7B2, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and produced recombinantly, researchers can expect uniform performance across lots, eliminating the variability often encountered with traditional hybridoma-derived antibodies. The rabbit IgG format, raised against a synthetic peptide from human DNA Ligase I, provides high specificity for detecting this essential nuclear enzyme.

Validation studies demonstrate reliable performance across multiple applications. In western blot analysis, the antibody detects LIG1 in both HeLa and HepG2 whole cell lysates at dilutions ranging from 1:500 to 1:5000. The observed band at approximately 140 kDa runs higher than the predicted molecular weights (102, 89, 99 kDa), a shift commonly attributed to post-translational modifications such as phosphorylation, or a significant enhancement of its molecular sieving effect in the SDS-PAGE gel and a noticeable slowdown in its migration speed resulting from the high content of proline in the LIG1 protein. Immunohistochemistry validation confirms nuclear staining patterns in paraffin-embedded human lung cancer and placenta tissues at 1:50 to 1:200 dilutions using standard citrate buffer antigen retrieval.

For researchers exploring epigenetics, nuclear signaling, or DNA repair pathways, this antibody provides a dependable tool for characterizing LIG1 expression and localization across diverse experimental contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Gene References into Functions

single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase PMID: 28751376
A histone H3K9-like mimic within LIG1 is methylated by G9a and GLP and avidly binds UHRF1. Interaction with methylated LIG1 promotes the recruitment of UHRF1 to DNA replication sites and is required for DNA methylation maintenance. PMID: 28803780
The rs156641 polymorphism of DNA ligase 1 (LIG1) was significantly associated with lung cancer risk, whereas no association was found between rs3730931/rs439132/rs20579 polymorphisms and lung cancer. PMID: 27352326
Data suggest that DNA ligase I (LigI)-deficient 46BR.1G1 cells represent a model to investigate the biological effects of sub-lethal levels of DNA insults. PMID: 26151554
The LIG1 CC genotype was associated with susceptibility to non-small cell lung cancer, and the AA genotype demonstrated increased radiosensitivity compared to the AC and CC genotypes. PMID: 26125914
A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells. PMID: 25879709
Ppolymorphisms in LIG1 affect its expression and may therefore change its function. PMID: 25189241
there is no association between LIGI polymorphisms and cervical cancer risk. However, they may be playing an important role in modulating the risk of cervical adenocarcinoma in North Indian women PMID: 24084463
DNA ligase I also interacts with replication factor C, the factor that loads the PCNA trimeric ring onto DNA. PMID: 22918593
Data indicate that Ku70/Ku80 facilitates the cooperative binding of multiple XRCC4/Ligase IV (XL) and XLF molecules to DNA. PMID: 23620595
Single nucleotide polymorphisms in LIG1 are associated with myelodysplastic syndromes. PMID: 23339595
phosphorylation of serine 51 on hLigI plays a critical role in regulating the interaction between hLigI and RFC, which is required for efficient DNA replication and repair. PMID: 22952233
Data show that association of study-wide significance (P < 8.2 x 10(-5)) was identified for single-nucleotide polymorphisms (SNP) in TP53, LIG1, and BIK. PMID: 22139380
Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency. PMID: 21561855
RNA silencing of human DNA ligase I expression severely reduced replication of viral DNA in cells infected with vaccinia virus ligase-deficient mutants. PMID: 20006844
DNA ligase I competes with FEN1 to expand repetitive DNA sequences in vitro. PMID: 11948189
stimulated by APE1 for progression through the base excision repair pathway PMID: 12200445
phosphorylation of DNA ligase I and possibly other replicative enzymes is part of the mechanism that directs the disassembly of the replication machinery at the completion of S-phase. PMID: 12851383
majority of single-strand DNA interruptions produced during the repair of alkylated DNA bases are repaired by the pathway mediated by Pol beta and either Lig I or Lig III PMID: 14627836
crystal structure of human DNA ligase I (residues 233 to 919) in complex with a nicked, 5' adenylated DNA intermediate PMID: 15565146
The human checkpoint sensor and alternative clamp Rad9-Rad1-Hus1 can interact with and specifically stimulate DNA ligase I PMID: 15871698
LIG1-deficiency reduces recombinational repair of DNA double-strand beaks. PMID: 15907772
human DNA ligase I is stimulated by the Rad9-rad1-Hus1 checkpoint complex PMID: 16731526
siRNA mediated down-regulation of DNA ligase I in human HTD114 cells led to impaired end joining that was mediated by 2-, 3- or 10-bp microhomology. PMID: 18440984
The LIG1 SNP 5'UTR showed a significant association with glioma risk. PMID: 19124499
drastically reduced replicative LigI activity in 46BR.1G1 cells results in the accumulation of both single-stranded and double-stranded DNA breaks. PMID: 19223467
a mutant version of hLigI, which mimics the hyperphosphorylated M-phase form of hLigI, does not interact with and is not inhibited by RFC, demonstrating that inhibition of ligation is dependent upon the interaction between hLigI and RFC PMID: 19223468
The DNA binding domain (DBD) within the hLigI catalytic fragment interacts with both PCNA and the heterotrimeric cell-cycle checkpoint clamp, hRad9-hRad1-hHus1 (9-1-1). PMID: 19523882
Data support the notion that DNA ligase I participates in homology dependent pathways that deal with replication-associated lesions generated when replication fork encounters DNA damage. PMID: 19597347
Data show that disruption of LigI and PCNA interactions influences trinucleotide repeat instability. PMID: 19628465

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Subcellular Location

Nucleus.

Protein Families

ATP-dependent DNA ligase family

Database Links

HGNC: 6598

OMIM: 126391

KEGG: hsa:3978

STRING: 9606.ENSP00000263274

UniGene: Hs.1770

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

DNA repair pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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