LPP Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA179059A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:HEK293 whole cell lysate(30µg), A431 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), Hela whole cell lysate(30µg), HepG2 whole cell lysate(30µg) All lanes: Lipoma preferred partner antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 66 kDa Observed band size: 75 kDa Exposure time：20s IHC image of CSB-RA179059A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA179059A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA179059A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

Q93052

Target Names

LPP

Alternative Names

DKFZp779O0231 antibody; FLJ30652 antibody; FLJ41512 antibody; LIM domain containing preferred translocation partner in lipoma antibody; LIM domain-containing preferred translocation partner in lipoma antibody; LIM protein antibody; Lipoma preferred partner antibody; Lipoma-preferred partner antibody; lpp antibody; LPP_HUMAN antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human LPP protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

23C5

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

LPP, or Lipoma-preferred partner, is a LIM domain-containing protein that plays a significant role in cell adhesion, migration, and cytoskeletal organization. Originally identified through its involvement in chromosomal translocations associated with lipomas, LPP has since emerged as an important player in signal transduction pathways, making it a valuable target for researchers investigating cellular dynamics, cancer biology, and tissue development.

This recombinant monoclonal antibody, clone 23C5, offers the reliability that comes with sequence-defined production. Because it derives from a single cloned sequence expressed in rabbit host cells, you can expect consistent performance across experiments and between lots, eliminating the variability that can complicate long-term studies or multi-site collaborations.

Validation data demonstrates robust performance across multiple applications. In western blot analysis, the antibody detects LPP in a diverse panel of human cell lines including HEK293, A431, COLO205, MCF7, HeLa, and HepG2, with an observed band at approximately 75 kDa. This migration slightly above the predicted 66 kDa molecular weight likely reflects post-translational modifications such as phosphorylation, which is consistent with LPP's known regulatory mechanisms in signaling pathways. For immunohistochemistry, the antibody has been validated in paraffin-embedded human kidney and liver cancer tissues, providing clear staining patterns suitable for tissue-based investigations. Flow cytometry validation using 786-O renal carcinoma cells confirms utility for single-cell analysis of intracellular LPP expression.

Whether you are exploring LPP's contributions to focal adhesion dynamics, investigating its role in tumor progression, or characterizing its expression patterns across tissue types, this antibody provides the flexibility and dependability needed for rigorous signal transduction research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.

Gene References into Functions

Data indicate a function for lipoma preferred partner (LPP) in the formation of invadopodia and a requirement for LPP in mediating the metastatic ability of breast cancer cells. PMID: 28436416
This is the first report of epigenetic regulation of the intronic miR-28-5p expression by promoter DNA methylation of its host gene, LPP. PMID: 28775176
meta-analysis provides robust estimates that polymorphisms in LPP and TAGAP genes are potential risk factors for celiac disease in Europeans and Americans PMID: 28208589
this study shows the importance of PP2A complexes with the LIM domains of lipoma-preferred partner in cell adhesion and migration dynamics PMID: 26945059
alteration of LPP expression markedly changes the collectiveness and metastatic potential of cancer cells PMID: 26028032
Results suggest that rs4686484 is the functional variant in this locus, while LPP expression is decreased in CeD. PMID: 24334606
3q28 rs6444305 in LPP gene is associated with follicular lymphoma. PMID: 25279986
interaction between LPP and alpha-actinin, an actin cross-linking protein, is necessary for TGFbeta-induced migration and invasion of ErbB2-expressing breast cancer cells. PMID: 23447672
Three polymorphisms of LPP gene were selected and replicated in additional 1132 PCOS cases and 1142 controls. Our results suggest that LPP gene might be a novel candidate for PCOS PMID: 23056290
The results suggest that genomic alterations and clearly deleterious sequence changes in the LPP gene are not a common cause of esophageal atresia/tracheoesophageal fistula or VACTERL association. PMID: 22639458
LPP is a nucleocytoplasmatic shuttle protein linking focal adhesion dynamics to the transcriptional machinery. PMID: 19701494
Gene expression levels of bcl-6, lpp and miR-28 are different in various diffuse large B cell lymphoma cell lines. PMID: 19236753
haploinsufficiency of LPP may be a novel cause of conotruncal cardiac anomalies, particularly forms of tetralogy of Fallot PMID: 20949626
LPP contains specific domains that enable its focal adhesion and nuclear targeting capacity PMID: 12441356
Expression of the HMGA2-LPP fusion transcript in only 1 of 61 karyotypically normal pulmonary chondroid hamartomas. PMID: 12505264
LPP and Scrib proteins localize in cell-cell contacts. This interaction links Scrib to a communication pathway between cell-cell contacts and the nucleus, and implicates LPP in Scrib-associated functions PMID: 15649318
Translocation in chromosome 3 and 12 involves fusion of this protein with HMGA2 in pulmonary chondroid hamartoma. PMID: 16271958
HMGA2-LPP fusion promotes chondrogenesis by upregulating cartilage-specific collagen gene expression through the N-terminal DNA binding domains. PMID: 16375854
LPP appears to be a vascular smooth muscle (SMSC) differentiation marker that plays a role in regulating SMC migration. PMID: 16397143
LPP can shuttle between the cytoplasm and the nucleus, suggesting they may transfer information directly from the cytoskeleton or focal adhesions to the transcription machinery. PMID: 16484626
By manipulating LPP levels, we show that it acts to upregulate the transactivation capacity of PEA3. PMID: 16738319
The function of LPP and palladin is context dependent, that they play a critical role in cytoskeletal remodeling, respond to signals induced by vascular injury as well as signals that induce smooth muscle cell hypertrophy, such as angiotension II. PMID: 17322171
expression of LPP and palladin are modulated by a mix of mechanical cues, oxidative stress and substrate composition which translate into their up or down regulation in vessel wall injury and early atherogenesis. PMID: 19205907

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Involvement in disease

A chromosomal aberration involving LPP is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with HMGA2 is shown in lipomas.; DISEASE: Note=A chromosomal aberration involving LPP is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with HMGA2 is detected in pulmonary chondroid hamartomas.; DISEASE: Note=A chromosomal aberration involving LPP is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with HMGA2 is also shown in one parosteal lipoma.; DISEASE: Note=A chromosomal aberration involving LPP is associated with acute monoblastic leukemia. Translocation t(3;11)(q28;q23) with KMT2A/MLL1 is associated with acute monoblastic leukemia.

Subcellular Location

Nucleus. Cytoplasm. Cell junction. Cell membrane. Note=Found in the nucleus, in the cytoplasm and at cell adhesion sites. Shuttles between the cytoplasm and the nucleus. It has been found in sites of cell adhesion such as cell-to-cell contact and focal adhesion which are membrane attachment sites of cells to the extracellular matrix. Mainly nuclear when fused with HMGA2/HMGIC and KMT2A/MLL1.

Protein Families

Zyxin/ajuba family

Tissue Specificity

Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes.

Database Links

HGNC: 6679

OMIM: 600700

KEGG: hsa:4026

STRING: 9606.ENSP00000318089

UniGene: Hs.720220

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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