Product Details

Uniprot No.

P09960

Target Names

LTA4H

Alternative Names

Leukotriene A-4 hydrolase (LTA-4 hydrolase) (EC 3.3.2.6) (Leukotriene A(4) hydrolase), LTA4H, LTA4

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human LTA4H

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6G7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the LTA4H recombinant monoclonal antibody is a complex process that involves several stages. Initially, the LTA4H monoclonal antibody is harvested and its gene sequence is determined. Then, a vector carrying the LTA4H monoclonal antibody gene is designed and transfected into a host cell line for culturing. During the LTA4H monoclonal antibody synthesis, a synthesized peptide based on human LTA4H is used as an immunogen. The LTA4H recombinant monoclonal antibody is then purified using affinity chromatography. Finally, the specificity of the LTA4H monoclonal antibody is evaluated using ELISA and WB assays to ensure that it is capable of recognizing its target. It reacts with human and mouse LTA4H proteins.

The LTA4H protein is an enzyme that belongs to the epoxide hydrolase family, and its activity is crucial in the biosynthesis of leukotrienes and lipoxins, which are potent lipid mediators involved in inflammation and immune responses. LTA4H plays a crucial role in regulating the inflammatory response in cells by catalyzing the conversion of LTA4 to either LTB4 or LXA4. LTA4H also plays a role in regulating the immune response by modulating the activation and differentiation of immune cells. LTA4H is also involved in regulating immune cell activation and differentiation, wound healing, and cancer development and progression.

Related Products

Target Background

Function

Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the proinflammatory mediator leukotriene B4 (LTB4). Has also aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its proinflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL). Involved also in the biosynthesis of resolvin E1 and 18S-resolvin E1 from eicosapentaenoic acid, two lipid mediators that show potent anti-inflammatory and pro-resolving actions.

Gene References into Functions

The structures reveal that a single catalytic water is involved in both catalytic activities of LTA4H, alternating between epoxide ring opening and peptide bond hydrolysis, assisted by E271 and E296, respectively. Moreover, we have found two conformations of LTA4H, uncovering significant domain movements. PMID: 28827365
A higher incidence of severe IRIS among patients with mutant LTA4H genotypes (CT and TT) was observed compared to the wild type, despite similar IRIS incidence and immune restoration in both groups. Steroids were effective in alleviating IRIS in all the genotypes PMID: 27643598
The LTA4H promoter polymorphism correlated with CSF mononuclear cell count but not with mortality in tuberculous meningitis PMID: 28419315
LTA4H genotype predicted survival of HIV-uninfected patients, with TT-genotype patients significantly more likely to survive tuberculous meningitis than CC-genotype patients. LTA4H genotype and HIV infection influence pretreatment inflammatory phenotype and survival from tuberculous meningitis. LTA4H genotype may predict adjunctive corticosteroid responsiveness in HIV-uninfected individuals. PMID: 28419368
LTA4H promoter region SNP was associated with susceptibility to bacteriologically confirmed bacterial meningitis but did not influence clinical presentation, disease severity or survival following dexamethasone treatment. PMID: 25799317
Report no association between LTA4H SNPs and atherosclerotic plaque phenotypes. PMID: 25721704
The best proteinogenic amino acid recognized by LTA4H is arginine. PMID: 24573245
The results of our study failed to confirm whether the selected variants in LTA4H gene within the LT metabolism pathway contribute to platelet reactivity in a diabetic population treated with ASA. PMID: 23828562
The LTA4H A-9188>G polymorphism has a strong association with severe asthma in children. PMID: 23573270
Data indicate that 13S,14S-epoxy-docosahexaenoic acid (DHA) inhibits leukotriene B4 (LTB4) biosynthesis by leukotriene A4 hydrolase (LTA4H). PMID: 23504711
We identified a novel leukotriene haplotype that appears to be protective toward subclinical atherosclerosis. This association is modified by dietary intake of polyunsaturated fatty acids PMID: 23153620
genetic association studies in population of Han Chinese in Eastern China: Using a recessive genetic model, an SNP in LTA4H (rs6538697) is associated with increased risk of ischemic stroke. PMID: 23079278
Structural origins for the loss of catalytic activities of bifunctional human LTA4H revealed through molecular dynamics simulations. PMID: 22848428
It was found modest association with LTA4H rs1978331C (intron 11) with increased FEV1 (p = 0.029) and with increased FEV1/FVC ratio (p = 0.020). PMID: 22206291
common polymorphisms in the LTA4H gene do not play any major role in susceptibility to clinical pulmonary tuberculosis PMID: 21112816
LTA4H and ALOX5AP gene polymorphisms modify the augmentation of bronchodilator responsiveness by leukotriene modifiers in Puerto Ricans but not Mexicans with asthma. PMID: 20810156
Our results support the role of LTA4H and ALOX5AP variants as risk factors for asthma in Latino populations. PMID: 20067482
Several point mutants of LTA4H with altered substrate specificities were designed. PMID: 20432426
In humans, protection from both tuberculosis and multibacillary leprosy is associated with heterozygosity for LTA4H polymorphisms that have previously been correlated with differential LTB(4) production. PMID: 20211140
The 5-lipoxygenase-leukotriene A4 pathway might play roles in the proliferation of human glioma cells. PMID: 19919819
5-lipoxygenase and leukotriene A4 hydrolase expression in atherosclerotic lesions correlates with symptoms of plaque instability PMID: 16698924
leukotriene A4 hydrolase These data provide evidence for the role of SNPs spanning the ALOX5AP and LTA4H genes in asthma and atopy susceptibility PMID: 18547289
Analysis of the thermodynamic parameters of irreversible thermal unfolding suggests that entropy-driven factors are responsible for the fast unfolding rate of the cold-adapted aminopeptidase. PMID: 18599387
Observational study and clinical trial of gene-environment interaction and pharmacogenomic / toxicogenomic. (HuGE Navigator) PMID: 15886380

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Subcellular Location

Cytoplasm.

Protein Families

Peptidase M1 family

Tissue Specificity

Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts.

Database Links

HGNC: 6710

OMIM: 151570

KEGG: hsa:4048

STRING: 9606.ENSP00000228740

UniGene: Hs.524648

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