Code | CSB-RA013425A0HU |
Size | US$210 |
Order now | |
Have Questions? | Leave a Message or Start an on-line Chat |
Immunize an animal with a human MAP3K3-derived peptide to stimulate an immune response. Isolate B cells from the immunized animal and fuse them with myeloma cells, creating hybridoma cells. Screen and select a single hybridoma cell clone that produces the desired MAP3K3-specific antibodies. Extract RNA from the selected hybridoma cells. Use reverse transcription PCR techniques to amplify and isolate the gene sequence coding for the MAP3K3 antibody's heavy and light chains. Insert the isolated MAP3K3 antibody genes into an expression vector, which is transfected into a host system for expression. Purify the MAP3K3 recombinant monoclonal antibodies using affinity chromatography from the cell culture supernatant. Confirm binding specificity and affinity of the recombinant monoclonal MAP3K3 antibody using ELISA. This antibody recognizes human MAP3K3 protein.
There are currently no reviews for this product.