MATR3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA221924A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: K562 whole cell lysate(30µg), NIH/3T3 whole cell lysate(30µg) All lanes: Matrin 3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 95 kDa Observed band size: 125 kDa Exposure time：1min IHC image of CSB-RA221924A0HU diluted at 1:100 and staining in paraffin-embedded human glioma cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA221924A0HU diluted at 1:100 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of SH-SY5Y cell with CSB-RA221924A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA221924A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P43243

Target Names

MATR3

Alternative Names

Matrin-3, MATR3, KIAA0723

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide from human MATR3 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

10C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Matrin-3 is a highly conserved nuclear matrix protein that plays essential roles in RNA processing, DNA replication, and transcriptional regulation. Mutations in MATR3 have been linked to amyotrophic lateral sclerosis (ALS) and distal myopathy, making it a protein of significant interest in neurodegenerative disease research and muscle biology studies.

This recombinant monoclonal antibody, clone 10C3, offers the consistency and reliability that demanding research applications require. Produced using recombinant technology with a sequence-defined approach, it eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies, ensuring reproducible results across long-term studies and multi-site collaborations.

Validation testing demonstrates robust performance across multiple experimental platforms. In western blot applications, the antibody detects MATR3 in both human K562 and mouse NIH/3T3 cell lysates, confirming cross-species reactivity between human and mouse samples. The observed band at approximately 125 kDa runs higher than the predicted 95 kDa molecular weight, which is consistent with known post-translational modifications of Matrin-3, including phosphorylation events that regulate its function. For immunohistochemistry, the antibody has been validated in human glioma and testis tissue sections using citrate buffer antigen retrieval. Immunofluorescence and flow cytometry applications have been successfully performed using SH-SY5Y neuroblastoma cells, a particularly relevant model for neurological research.

The antibody is supplied in a glycerol-containing buffer optimized for long-term storage stability. Whether investigating MATR3's role in RNA metabolism, exploring its involvement in neurodegenerative pathways, or examining its function in nuclear architecture, this antibody provides a dependable tool for advancing your research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. In association with the SFPQ-NONO heteromer may play a role in nuclear retention of defective RNAs. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway. May bind to specific miRNA hairpins.

Gene References into Functions

Depletion of SAFB1 reduced FUS's localization to chromatin-bound fraction and splicing activity, suggesting SAFB1 could tether FUS to chromatin compartment thorough N-terminal DNA-binding motif. Moreover, FUS interacts with another nuclear matrix-associated protein, Matrin3. PMID: 27731383
Using a novel RNA pulldown approach that utilized endogenous S1-tagged PINCR (a p53-regulated long noncoding RNA), the authors show that PINCR associates with the enhancer region of these genes by binding to RNA-binding protein Matrin 3 that, in turn, associates with p53. PMID: 28580901
Matrin3 physically interacts with intronic pyrimidine-rich sequences and controls alternative splicing. PMID: 28695676
It may not be a common genetic factor in Chinese amyotrophic lateral sclerosis patients. PMID: 26708275
Its mutations strengthens the role of RNA metabolism in amyotrophic lateral sclerosis etiology. PMID: 26493020
A missense mutation in MATR3 was identified in myopathy patients undergoing a needle electromyography. PMID: 26899464
Our findings indicate that mutations in Matrin 3 that are associated with ALS and myopathy do not dramatically alter the normal localization of the protein or readily induce inclusion formation. PMID: 26528920
Three-dimensional mapping of the lamin A-matrin-3 interface showed that the LMNA truncating mutation Delta303, which lacks the matrin-3 binding domain, was associated with an increased distance between lamin A and matrin-3. PMID: 25948554
Suppressing Matrin 3 powers a heightened and broader ZAP restriction of HIV-1 gene expression. PMID: 26129669
MATR3 mutation is identified to be a possible cause of amyotrophic lateral sclerosis. PMID: 25771394
No mutations were identified, indicating that MATR3 mutations are not a common cause of Amyotrophic lateral sclerosis in Australian familial cases with predominately European ancestry PMID: 25523636
MATR3 gene disruption is associated with bicuspid aortic valve, aortic coarctation and patent ductus arteriosus. PMID: 25574029
Mutations in MATR3 are rare in French familial ALS and ALS with FTLD patients. PMID: 25158920
This study shows a high risk of abnormal respiratory function with progressive worsening in MATR3 myopathy. PMID: 25677933
Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB. PMID: 25599992
the pY RNA1-s2/Matr3 interaction could play a role in vision PMID: 24558381
Sixteen patients from 6 families with late onset distal myopathy associated with the p.S85C MATR3 mutation were characterized. PMID: 25154462
This study identified mutations in MATR3 in ALS kindreds. also observed MATR3 pathology in ALS-affected spinal cords with and without MATR3 mutations. PMID: 24686783
Data suggest that the cellular level of MATR3, known to be highly regulated, modulates the stability of a group of gene transcripts. PMID: 21858232
Rev needs MATR3 to promote the cytoplasmic accumulation and translation of unspliced RRE-containing mRNA. PMID: 21771348
MATR3 binds viral RNA and is required for the Rev/RRE mediated nuclear export of unspliced HIV-1 RNAs. PMID: 21771346
Matrin 3 binds Rev RNA to stabilize HIV-1 transcripts leading to increased cytoplasmic expression. PMID: 21771347
MATR3 to be a novel ATM target in response to DNA damage. PMID: 20421735
matrin 3 plays a significant role in controlling cell growth and proliferation PMID: 21182838
study of the association of matr3 with chromosome territories and identification of potential interacting proteins PMID: 19562669
Data show that the nuclear matrix protein matrin 3, cytoskeletal motor protein HMP, and the circadian clock protein lark were significantly decreased in fetal Down syndrome brain. PMID: 12469345
These results suggest that the functions of matrin 3 could be regulated by both Ca(2+)-dependent interaction with calmodulin and caspase-mediated cleavage. PMID: 17658460
the spatial proximities among a constellation of functionally related sites that are found within euchromatic regions of the cell nucleus including: HP1gamma, RNA polymerase II, matrin 3, and SAF-A sites PMID: 18618731
nonconservative S85C missense mutation in vocal cord and pharyngeal weakness with distal myopathy PMID: 19344878

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Involvement in disease

Amyotrophic lateral sclerosis 21 (ALS21)

Subcellular Location

Nucleus matrix.

Database Links

HGNC: 6912

OMIM: 164015

KEGG: hsa:9782

STRING: 9606.ENSP00000354346

UniGene: Hs.268939

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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