Code | CSB-RA276759A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
The MDK recombinant monoclonal antibody is produced using a combination of protein and DNA recombinant technology. Initially, mice were given an immunization with a synthesized peptide that was derived from human midkine. After that, the spleen was aseptically removed from the mice and the RNA from the spleen cells was extracted. And cDNA was created through the RNA reverse transcription and used as a template for the PCR amplification of the MDK antibody gene. The resulting MDK antibody gene was then introduced into a vector, which was then transfected into host cells for culture. The MDK recombinant monoclonal antibody was then purified from the supernatant of cell culture via affinity chromatography. It has been verified and can be utilized for human MDK protein detection in ELISA and IHC experiments.
Midkine (MDK) is involved in various cellular processes, including development, tissue repair, and inflammation. It is highly expressed during embryonic development and is involved in tissue morphogenesis and organogenesis. It plays a crucial role in cell proliferation, migration, and survival. MDK has been implicated in angiogenesis by promoting endothelial cell proliferation and migration. Its dysregulation has been associated with several pathological conditions, including cancer and inflammatory diseases.
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