Code | CSB-RA242999A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The MEN1 recombinant monoclonal antibody is a valuable tool for detecting human MEN1 protein in ELISA and WB applications. This antibody is produced using recombinant DNA technology. First, the cDNA of the MEN1 antibody-producing hybridomas is sequenced, and the gene coding for the MEN1 monoclonal antibody is synthesized. The hybridomas are formed by fusing B cells from an animal that was immunized with a synthesized peptide derived from human MEN1, with myeloma cells. The synthesized gene is then cloned into a vector. The recombinant vector is transfected into cells for cultivation. Finally, the resulting MEN1 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography.
The MEN1 protein, also known as menin, is a tumor suppressor protein that helps regulate the expression of various genes involved in cell growth and division. Specifically, menin interacts with a variety of transcription factors, including those involved in regulating the cell cycle and DNA repair, and modulates their activity to prevent uncontrolled cell growth and division. Mutations in the MEN1 gene can lead to the development of multiple endocrine neoplasia type 1, a rare inherited disorder characterized by tumors of the endocrine glands.
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