Product Details

Uniprot No.

Target Names

Alternative Names

adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase antibody; IME4 antibody; M6A antibody; Methyltransferase like protein 3 antibody; Methyltransferase-like protein 3 antibody; METTL3 antibody; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase antibody; mRNA m(6)A methyltransferase antibody; MT-A70 antibody; MTA70 antibody; MTA70_HUMAN antibody; N6 adenosine methyltransferase 70 kDa subunit antibody; N6-adenosine-methyltransferase 70 kDa subunit antibody

Species Reactivity

Human, Mouse

Immunogen

A synthesized peptide derived from human METTL3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6D10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

METTL3 serves as the catalytic core of the N6-methyladenosine (m6A) writer complex, the most abundant internal modification found in eukaryotic messenger RNA. This methyltransferase plays a fundamental role in post-transcriptional gene regulation, influencing mRNA stability, splicing, nuclear export, and translation efficiency. Given its emerging connections to stem cell differentiation, circadian rhythm regulation, and oncogenic pathways, METTL3 has become a compelling target for researchers investigating epitranscriptomic mechanisms in both normal physiology and disease states.

This recombinant monoclonal antibody, generated from clone 6D10 in rabbit host, offers the reproducibility essential for longitudinal studies of m6A biology. Because the antibody sequence is defined and production occurs through recombinant expression rather than hybridoma culture, researchers benefit from consistent performance across experiments and minimal lot-to-lot variation, a critical consideration when comparing results over time or across collaborating laboratories.

Validation by western blot demonstrates reliable detection across multiple human cell lines including Jurkat, HT-29, HeLa, and K562, as well as mouse NIH/3T3 cells, confirming cross-species reactivity between human and mouse samples. The observed band at approximately 70 kDa runs slightly higher than the predicted 64 kDa molecular weight, consistent with post-translational modifications commonly observed for this protein. Immunohistochemical staining has been validated in paraffin-embedded human lung cancer tissue using citrate buffer antigen retrieval, with clear signal achieved at 1:100 dilution.

This antibody supports researchers exploring m6A-mediated gene regulation in cancer biology, developmental processes, and metabolic disease, providing a dependable tool for interrogating METTL3 expression and localization across diverse experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some RNAs and regulates various processes such as the circadian clock, differentiation of embryonic and hematopoietic stem cells, cortical neurogenesis, response to DNA damage, differentiation of T-cells and primary miRNA processing. In the heterodimer formed with METTL14, METTL3 constitutes the catalytic core. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability, processing, translation efficiency and editing. M6A acts as a key regulator of mRNA stability: methylation is completed upon the release of mRNA into the nucleoplasm and promotes mRNA destabilization and degradation. In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization, promoting differentiation of ESCs. M6A regulates the length of the circadian clock: acts as an early pace-setter in the circadian loop by putting mRNA production on a fast-track for facilitating nuclear processing, thereby providing an early point of control in setting the dynamics of the feedback loop. M6A also regulates circadian regulation of hepatic lipid metabolism. M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis. Also required for oogenesis. Involved in the response to DNA damage: in response to ultraviolet irradiation, METTL3 rapidly catalyzes the formation of m6A on poly(A) transcripts at DNA damage sites, leading to the recruitment of POLK to DNA damage sites. M6A is also required for T-cell homeostasis and differentiation: m6A methylation of transcripts of SOCS family members (SOCS1, SOCS3 and CISH) in naive T-cells promotes mRNA destabilization and degradation, promoting T-cell differentiation. Inhibits the type I interferon response by mediating m6A methylation of IFNB. M6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs). Mediates m6A methylation of Xist RNA, thereby participating in random X inactivation: m6A methylation of Xist leads to target YTHDC1 reader on Xist and promote transcription repression activity of Xist. M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells. METTL3 mediates methylation of pri-miRNAs, marking them for recognition and processing by DGCR8. Acts as a positive regulator of mRNA translation independently of the methyltransferase activity: promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate in cancer cell proliferation by promoting mRNA translation. During human coronorivus SARS-CoV-2 infection, adds m6A modifications in SARS-CoV-2 RNA leading to decreased DDX58/RIG-I binding and subsequently dampening the sensing and activation of innate immune responses.

Gene References into Functions

Findings suggest that METTL3 plays very important oncogenic roles in ovarian carcinoma development and/or aggressiveness by stimulating AXL translation and EMT. PMID: 30249526
Data show that hepatitis B X-interacting protein (HBXIP) modulated Methyltransferase-like 3 (METTL3) by inhibiting miRNA let-7g, which down-regulated the expression of METTL3 by targeting its 3'UTR. PMID: 29174803
Study reports the importance of m6A modification in glioma stem-like cells and uncovers METTL3 as a potential molecular target in glioblastoma therapy. PMID: 28991227
The role of METTL3 in pancreatic cancer cells drug resistance and radiation resistance.METTL3 was associated with mitogen-activated protein kinase cascades, ubiquitin-dependent process and RNA splicing and regulation of cellular process. PMID: 29345285
METTL3 is soluble and inactive while the catalytic center of METTL14 is degenerated and thus also inactive. In addition, the C-terminal RGG repeats of METTL14 are required for METTL3/14 activity by contributing to RNA substrate binding. PMID: 29348140
data define METTL3 as a regulator of a chromatin-based pathway that is necessary for maintenance of the leukaemic state and identify this enzyme as a potential therapeutic target for acute myeloid leukaemia PMID: 29186125
loss of METTL3 leads to increased levels of phosphorylated AKT, which contributes to the differentiation-promoting effects of METTL3 depletion. Overall, these results provide a rationale for the therapeutic targeting of METTL3 in myeloid leukemia PMID: 28920958
The structure reveals the heterodimeric architecture of the complex and donor substrate binding by METTL3. Structure-guided mutagenesis indicates that METTL3 is the catalytic subunit of the complex, whereas METTL14 has a degenerate active site and plays non-catalytic roles in maintaining complex integrity and substrate RNA binding. PMID: 27627798
For the methylation of adenosine in RNA, Mettl3 is the catalytically active subunit, while Mettl14 plays a structural role critical for substrate recognition. PMID: 27373337
METTL3 enhances mRNA translation through an interaction with the translation initiation machinery in lung adenocarcinoma cells. PMID: 27117702
miR-33a can attenuatenon-small-cell lung carcinoma cells proliferation via targeting to the 3'-untranslated region of METTL3 mRNA. PMID: 27856248
Structure of the METTL3-METTL14 complex PMID: 27281194
genetic inactivation impaired embryonic stem cells differentiation PMID: 25456834
Study identifies m(6)A methylation sites on many clock gene transcripts and shows that specific inhibition of m(6)A methylation by silencing of the m(6)A methylase Mettl3 is sufficient to elicit circadian period elongation and RNA processing delay. PMID: 24209618

Hide All

Subcellular Location

Nucleus. Nucleus speckle. Cytoplasm.

Protein Families

MT-A70-like family

Tissue Specificity

Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.

Database Links

HGNC: 17563

OMIM: 612472

KEGG: hsa:56339

STRING: 9606.ENSP00000298717

UniGene: Hs.168799

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Code	CSB-RA559330A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Jurkat whole cell lysate(30µg),HT-29 whole cell lysate(30µg),Hela whole cell lysate(30µg),K562 whole cell lysate(30µg),NIH/3T3 whole cell lysate(30µg) All lanes: METTL3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 64 kDa Observed band size: 70 kDa Exposure time：2min IHC image of CSB-RA559330A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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METTL3 Recombinant Monoclonal Antibody

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