MGST1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA906984A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(30µg), HeLa whole cell lysate(30µg), A431 whole cell lysate(30µg), PC-3 whole cell lysate(30µg), U-251MG whole cell lysate(30µg), Mouse liver tissue lysate(30µg), Rat liver tissue lysate(30µg) All lanes: MGST1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa Exposure time：2min IHC image of CSB-RA906984A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA906984A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing 786-O cells stained with CSB-RA906984A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

P10620

Target Names

MGST1

Alternative Names

Microsomal glutathione S-transferase 1 (Microsomal GST-1) (EC 2.5.1.18) (Microsomal GST-I), MGST1, GST12 MGST

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human MGST1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

12H11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Microsomal glutathione S-transferase 1 (MGST1) serves as a critical enzyme in cellular detoxification pathways, catalyzing the conjugation of glutathione to electrophilic compounds and protecting cells against oxidative stress and xenobiotic damage. This membrane-associated protein is particularly abundant in liver tissue and plays significant roles in drug metabolism research, toxicology studies, and investigations into cellular stress responses and cancer biology.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human MGST1, offers the reproducibility and consistency that demanding research applications require. As a sequence-defined reagent produced through recombinant technology, it eliminates the lot-to-lot variability that can complicate long-term studies and ensures reliable performance across your experimental timeline.

Validation testing demonstrates robust performance across multiple platforms. Western blot analysis confirms specific detection of the expected 18 kDa band across a diverse panel of human cell lines including HepG2, HeLa, A431, PC-3, and U-251MG, with cross-species reactivity confirmed in mouse and rat liver tissue lysates. This broad species compatibility supports comparative studies and translational research workflows. Immunohistochemical staining has been validated in paraffin-embedded human liver cancer and stomach tissue sections using citrate buffer antigen retrieval, while flow cytometry applications have been demonstrated in 786-O renal carcinoma cells with clear positive population shifts.

Whether you are investigating hepatic detoxification mechanisms, exploring MGST1 expression patterns in tumor microenvironments, or characterizing stress response pathways across model systems, this antibody provides the flexibility to move seamlessly between detection platforms while maintaining the consistency your research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.

Gene References into Functions

High MGST1 expression is associated with Bendamustine-resistance in Mantle Cell Lymphoma. PMID: 29695404
Significant association for germline variations only in the COX pathway (specifically in the antioxidant microsomal glutathione S-transferase 1 [MGST1] gene), with risk for Barrett's esophagus and the combined outcome of Barrett's esophagus and esophageal adenocarcinoma was found. PMID: 29713984
This study provides the most comprehensive evaluation of inflammation-related germline variation in relation to risk of BE/OA and suggests that variants in MGST1 influence disease susceptibility. PMID: 27486097
The MSGT1 displays a preexisting dynamic equilibrium between high- and low-activity forms. PMID: 28558199
possibly marked variations in NB mitochondrial homeostasis, they also imply that the results of these earlier studies using NB cells are not transferable to other tumor and cell types that express MGST1 at high concentrations. PMID: 24486338
Differential expression of SOD2, ALDH1A1, and MGST1 genes in the anterior lens capsules of patients with pseudoexfoliation syndrome suggest that diseased tissue appears to respond to the previously reported oxidative stress. PMID: 23805041
The results suggest that MGST1 gene polymorphisms as one of environment gene may contribute to colorectal cancer risk in younger ages. PMID: 23314968
MGST1 protects cells (and mitochondria) by both conjugation and glutathione peroxidase functions. A new protective mechanism against cisplatin is also indicated PMID: 20727966
Polymorphisms in microsomal glutathione s transferase is associated with laryngeal cancer PMID: 12359356
The results indicate that Sp1 is the protein mediating the basal transcription of MGST1 PMID: 12818425
Genes encoding KU70, MGST1 and BIK show age-related mRNA expression levels in hematopoietic stem cells. PMID: 17714764
As an addition to the high-density SNP (single-nucleotide polymorphism) maps of 12 glutathione S-transferase and related genes reported earlier, a SNP map of the microsomal glutathione S-transferase 1 (MGST1) gene is provided. PMID: 11587073

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Subcellular Location

Microsome. Mitochondrion outer membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

MAPEG family

Tissue Specificity

Highly expressed in liver.

Database Links

HGNC: 7061

OMIM: 138330

KEGG: hsa:4257

STRING: 9606.ENSP00000010404

UniGene: Hs.389700

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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