MICA/B Recombinant Monoclonal Antibody

MICA and MICB are stress-inducible ligands for the NKG2D receptor, playing a central role in immune surveillance against tumors and virally infected cells. These MHC class I-related proteins are upregulated in response to cellular stress, DNA damage, and malignant transformation, making them critical markers in cancer immunology and infectious disease research. Understanding MICA/B biology has become increasingly important as researchers explore NKG2D-based immunotherapeutic strategies.

This recombinant monoclonal antibody, clone 5B8, offers the consistency and reproducibility that demanding quantitative assays require. Because it is produced from a defined sequence rather than hybridoma culture, you can expect uniform performance across lots, eliminating a common source of experimental variability in longitudinal studies or multi-site collaborations. The human IgG1 isotype format provides flexibility for researchers working on functional studies or developing detection systems.

Validation data demonstrates exceptional binding performance in ELISA applications, with EC50 values in the sub-nanogram to low nanogram range across multiple MICA and MICB protein variants. The antibody recognizes both human MICA and MICB with high affinity, and importantly, cross-species reactivity with Macaca fascicularis MICB has been confirmed through functional binding assays. This dual-species capability is particularly valuable for researchers conducting preclinical studies that require translation between non-human primate models and human samples.

The recommended ELISA dilution range of 1:5000 to 1:50000 reflects the antibody's strong binding characteristics, allowing you to optimize signal-to-background ratios while conserving reagent. Whether you are investigating tumor immune evasion mechanisms, characterizing stress response pathways, or developing NKG2D-targeted therapeutics, this antibody provides a reliable tool for quantitative MICA/B detection.