MRAS Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA014781MA1HU
Size	US$210
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Image	IHC image of CSB-RA014781MA1HU diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela cell with CSB-RA014781MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Target Background

Product Details

Uniprot No.

O14807

Target Names

MRAS

Alternative Names

FLJ42964 antibody; M ras antibody; M-ras antibody; Mras antibody; Muscle and microspikes Ras antibody; Muscle RAS oncogene homolog antibody; Muscle Ras oncogene homologue antibody; Muscle Ras viral oncogene homolog antibody; R ras3 antibody; R-ras3 antibody; Ras related protein MRas antibody; Ras related protein RRas3 antibody; Ras-related protein M-Ras antibody; Ras-related protein R-Ras3 antibody; RASM_HUMAN antibody; Related Ras viral oncogene homolog 3 antibody; RRas3 antibody; XRas antibody

Species Reactivity

Human

Immunogen

Recombinant Human MRAS protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse IgG2a

Clone No.

17H12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

MRAS, also known as R-Ras3, is a member of the Ras superfamily of small GTPases that plays a distinctive role in cellular signaling networks. Unlike its more extensively studied relatives, MRAS functions as a modulator of MAP kinase pathways and has been implicated in cell adhesion, migration, and differentiation processes. Its involvement in signal transduction cascades that influence cell fate decisions makes it a compelling target for researchers investigating oncogenic mechanisms and developmental biology.

This recombinant monoclonal antibody, generated from clone 17H12, offers the reproducibility and consistency that demanding experimental workflows require. Because recombinant antibodies are produced from defined sequences rather than hybridoma supernatants, researchers can expect uniform performance across lots, eliminating a common source of experimental variability. The antibody was raised against recombinant human MRAS protein and has been validated for human sample detection.

Experimental validation demonstrates versatility across multiple detection platforms. Immunohistochemistry performed on paraffin-embedded human prostate tissue using a Leica Bond system with citrate buffer antigen retrieval shows specific staining at dilutions ranging from 1:50 to 1:200. Immunofluorescence studies in HeLa cells reveal clear signal when used at 1:200 dilution, with the antibody successfully detecting MRAS in formaldehyde-fixed samples. These validated protocols provide researchers with established starting points for their own investigations.

The affinity-purified IgG2a format and glycerol-stabilized formulation support long-term storage stability at -20°C or -80°C. For investigators exploring cancer biology or signal transduction mechanisms, this antibody provides a reliable tool for examining MRAS expression patterns and localization in human tissues and cell culture models.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Serves as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Activates the MAP kinase pathway.

Gene References into Functions

acute coronary syndrome in Czechs (30.4% vs 29.4% carriers of the minor T allele of MRAS[recessive model], p = 0.54; OR 1.05; 95% CI 0.89-1.24 for males and 32.1% vs 29.7% carriers of the minor T allele, p = 0.28; OR 1.12; 95% CI 0.91-1.37 for females) PMID: 29264877
Results discovered for the first time that MRAS is recurrently mutated, indicating that MRAS mutations could drive tumorigenesis of Type IV gastric neoplasm. PMID: 27891760
The association of the MARS rs6782181 polymorphism and serum lipid levels is different between the Mulao and Han populations, or between males and females in the both ethnic groups. PMID: 25973078
The MRAS gene loci might have a minor effect in conferring susceptibility to coronary artery disease in the Chinese population. PMID: 25800439
Both MRAS and SHOC2 play a key role in polarized migration. PMID: 24211266
implicated in a novel pathway of neuronal differentiation by coupling specific trophic factors to the MAPK cascade through the activation of B-Raf PMID: 12138204
These findings proved a crucial role of the cross-talk between two Ras-family GTPases M-Ras and Rap1, mediated by RA-GEF-2, in adhesion signaling. PMID: 17538012
Identification of one new CAD risk locus on 3q22.3 in MRAS, and suggestive association with a locus on 12q24.31 near HNF1A-C12orf43. PMID: 19198612

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Subcellular Location

Cell membrane; Lipid-anchor; Cytoplasmic side.

Protein Families

Small GTPase superfamily, Ras family

Tissue Specificity

Expression highly restricted to the brain and heart.

Database Links

HGNC: 7227

OMIM: 608435

KEGG: hsa:22808

STRING: 9606.ENSP00000289104

UniGene: Hs.527021

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Related pathways

MAPK signaling pathway Regulation of actin cytoskeleton Autophagy Cellular senescence Ras signaling pathway Apelin signaling pathway Phospholipase D signaling pathway Rap1 signaling pathway Mitophagy Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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