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| Code | CSB-RA158563A0HU |
| Size | US$210 |
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| Application | Recommended Dilution |
|---|---|
| WB | 1:500-1:2000 |
CUSABIO meticulously developed the MRAS recombinant monoclonal antibody using a systematic approach. Initially, B cells were isolated from the spleen of an immunized animal, utilizing a synthesized peptide derived from human MRAS as the immunogen. Subsequently, RNA was extracted from the B cells and converted into cDNA through reverse transcription. The gene encoding the MRAS antibody was amplified using a degenerate primer and inserted into a vector. The recombinant vector was then introduced into host cells through transfection, allowing for efficient expression of the MRAS recombinant monoclonal antibodies. These antibodies were harvested from the cell culture supernatant and purified via affinity chromatography. This antibody can react with human MRAS protein and has been validated for use in ELISA and WB applications.
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