MRPL28 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA246132A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:HepG2 whole cell lysate(30µg), COLO205 whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Hela whole cell lysate(30µg), Jurkat whole cell lysate(30µg), MCF7 whole cell lysate(30µg), Mouse kidney tissue lysate(30µg), Rat kidney tissue lysate(30µg) All lanes: CtBP2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 49 kDa Observed band size: 49 kDa Exposure time：10s Overlay Peak curve showing Hela cells stained with CSB-RA246132A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q13084

Target Names

MRPL28

Alternative Names

39S ribosomal protein L28 antibody; 39S ribosomal protein L28 mitochondrial antibody; 39S ribosomal protein L28 mitochondrial precursor antibody; HGNC6756 antibody; L28mt antibody; MAAT 1 antibody; MAAT1 antibody; Melanoma antigen p15 antibody; Melanoma associated antigen recognised by cytotoxic T lymphocytes antibody; Melanoma associated antigen recognized by T lymphocytes antibody; Melanoma-associated antigen recognized by T-lymphocytes antibody; MGC8499 antibody; mitochondrial antibody; Mitochondrial ribosomal protein L28 antibody; MRP L28 antibody; MRP-L28 antibody; MRPL 28 antibody; mrpl28 antibody; P15 antibody; RM28_HUMAN antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human MRPL28 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

14B4

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Subcellular Location

Mitochondrion.

Protein Families

Bacterial ribosomal protein bL28 family

Tissue Specificity

Found in a variety of normal tissues including spleen, testes, thymus, liver, kidney, brain, adrenal, lung and retinal tissue.

Database Links

HGNC: 14484

OMIM: 604853

KEGG: hsa:10573

STRING: 9606.ENSP00000199706

UniGene: Hs.513230

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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