NDUFS3 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA224121A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, All lanes: NDUFS3 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31, 15 KDa Observed band size: 31 kDa IHC image of CSB-RA224121A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

O75489

Target Names

NDUFS3

Alternative Names

NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (EC 1.6.5.3) (EC 1.6.99.3) (Complex I-30kD) (CI-30kD) (NADH-ubiquinone oxidoreductase 30 kDa subunit), NDUFS3

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human NDUFS3

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1H12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NDUFS3 serves as a core subunit of mitochondrial Complex I, the largest enzyme complex in the electron transport chain responsible for initiating oxidative phosphorylation. This 30 kDa iron-sulfur protein plays an essential role in cellular energy metabolism, and its dysfunction has been implicated in mitochondrial disorders, metabolic reprogramming in cancer, and broader questions of cellular bioenergetics. For researchers investigating these pathways, reliable detection of NDUFS3 provides critical insight into mitochondrial function and integrity.

This recombinant monoclonal antibody, generated against a synthetic peptide derived from human NDUFS3, offers the consistency and reproducibility that demanding experimental workflows require. As a sequence-defined reagent produced from a single rabbit clone (1H12), it eliminates the lot-to-lot variability that can complicate longitudinal studies or multi-site collaborations. The affinity-purified IgG format ensures minimal background while maintaining strong target recognition.

Validation studies demonstrate robust performance across multiple applications. In western blot analysis, the antibody detects a clear band at the expected 31 kDa molecular weight in both 293 and HepG2 whole cell lysates, with effective detection at 1:2000 dilution. For tissue-based studies, immunohistochemistry validation in paraffin-embedded human kidney tissue confirms reliable staining using standard heat-induced epitope retrieval in citrate buffer, with recommended dilutions between 1:50 and 1:200. The antibody is also validated for ELISA applications.

Whether you are exploring mitochondrial dysfunction in cancer metabolism, characterizing Complex I assembly, or investigating signal transduction pathways linked to cellular energetics, this antibody provides a dependable tool for detecting NDUFS3 in human samples across your experimental platforms.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.

Gene References into Functions

identified a novel Leigh syndrome causing NDUFS3 mutation and expanded the clinical spectrum caused by NDUFS3 mutations in this study. PMID: 30140060
The results suggest that the impaired mitochondrial activity could be due to the broken interaction between DJ-1 and NDUFS3 and that downregulation of DJ-1 in sperm and testes contributes to AS pathogenesis. PMID: 29849492
The NDUFS3 expressed in the temporal cortex in patient with late-onset Alzheimer's disease. PMID: 28242297
High NDUFS3 expression is associated with IgA nephropathy. PMID: 25770168
pH stability of w-t and Leigh syndrome mutant varied at extreme acidic pH. the molten globule like structure of w-t at pH1 was absent in case of the mutant protein. Both the w-t and mutant proteins showed multi-step thermal and Gdn-HCl induced unfolding PMID: 24028823
NDUFS3 is down-regulated in serous ovarian adenocarcinoma PMID: 23446378
Expressions of GRIM-19, NDUFS3, and extracellular matrix elements are correlated with invasive capabilities of breast cancer cell lines. PMID: 23630608
a novel, biomarker potential of a mitochondrial complex I subunit protein, NDUFS3 - as a robust indicator of breast cancer progression and invasiveness as well as of hypoxia/necrosis in clinical specimens of invasive ductal carcinoma, was uncovered. PMID: 21867691
Decreased protein levels of complex I 30-kDa subunit in fetal Down syndrome brains. PMID: 11771736

Hide All

Subcellular Location

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Protein Families

Complex I 30 kDa subunit family

Database Links

HGNC: 7710

OMIM: 603846

KEGG: hsa:4722

STRING: 9606.ENSP00000263774

UniGene: Hs.502528

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Oxidative Phosphorylation

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address