Product Details

Uniprot No.

Target Names

NME1

Alternative Names

Nucleoside diphosphate kinase A (NDK A) (NDP kinase A) (EC 2.7.4.6) (Granzyme A-activated DNase) (GAAD) (Metastasis inhibition factor nm23) (NM23-H1) (Tumor metastatic process-associated protein), NME1, NDPKA NM23

Species Reactivity

Human

Immunogen

A synthesized peptide from human NME1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

4B12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NME1, also known as nucleoside diphosphate kinase A or NM23-H1, plays a fascinating dual role in cellular biology. Beyond its enzymatic function in nucleotide metabolism, NME1 has garnered significant attention as a metastasis suppressor gene, with its expression levels inversely correlating with tumor aggressiveness across multiple cancer types. This makes NME1 an important target for researchers investigating cancer progression, metastatic potential, and the molecular mechanisms underlying tumor spread.

This recombinant monoclonal antibody, generated against a synthetic peptide from human NME1, offers the consistency and reproducibility that demanding research applications require. As a sequence-defined recombinant clone, it eliminates the lot-to-lot variability inherent in traditional hybridoma-derived antibodies, ensuring your experimental results remain comparable across extended studies and collaborative projects.

Validation data demonstrates reliable performance across multiple platforms. Western blot analysis detects a clean 17 kDa band matching the predicted molecular weight in A549 lung carcinoma, HeLa cervical cancer, and MCF-7 breast cancer cell lysates, confirming specificity across diverse human cancer cell models. Immunohistochemistry staining in paraffin-embedded human colorectal cancer tissue shows effective antigen detection using standard citrate buffer retrieval methods. Flow cytometry analysis in SH-SY5Y neuroblastoma cells further extends its utility for single-cell studies, with clear separation from isotype control demonstrating specific intracellular detection.

This antibody serves researchers exploring cancer metastasis mechanisms, nucleotide metabolism, and tumor biology. Its validated performance in Western blot, immunohistochemistry, and flow cytometry provides workflow flexibility for characterizing NME1 expression across experimental contexts, from cell line screening to clinical tissue analysis.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.

Gene References into Functions

NME1 enhanced ALDOC transcription, evidenced by increased expression of ALDOC pre-mRNA and activity of an ALDOC promoter-luciferase module. This is the first study to indicate that NME1 induces transcription through its direct binding to the promoter region of a target gene. PMID: 30396920
NME1 rs16949649 associated with increased susceptibility to gynecological cancer and rs2302254 was linked to reduced gastric cancer risk (Meta-Analysis) PMID: 29525404
Immunohistochemical expression of nm23H1 is not an effective tool to distinguish among the cases of BPH, adenocarcinoma of prostate with and without metastasis. Hence nm23H1 gene does not behave like an antimetastatic gene in prostatic lesions. PMID: 29567887
The positive expression rates of KAI1 and nm23 were significantly lower in laryngeal squamous cell carcinoma than normal laryngeal mucosa. PMID: 29187211
coexistence of high MACC1 and low NM23-H1 expression and tumor budding was associated with short overall survival PMID: 29700912
elevated NDKA was associated with severe characteristics of adenomas (>/=3 lesions, size >/= 1 cm or villous component). Setting specificity to 85%, NDKA showed a sensitivity of 30.19% and 29.82% for advanced adenomas and advanced neoplasia, respectively. PMID: 27222072
This study suggested that EGFR was an important predictive factor for the prognosis of the post-operative patients with colorectal carcinoma TNM stage I-II, and nm23 is important for predicting the prognosis of the patients with stage III-IV; it is better that EGFR and nm23 are as predictor of combination. PMID: 27888614
Results demonstrate that nm23 plays a vital role in decidualization in mice and humans and that nm23 gene expression is hormonally regulated. PMID: 27604954
NM23 might be an indicator of good prognosis in patients with breast cancer, although further researches need to be performed to confirm the prognostic value of NM23. [Meta-analysis; review] PMID: 28161101
The data presented suggest an important role for cytoplasmic IRF6 in regulating the availability or localization of the NME1/2 complex and thus the dynamic behavior of epithelia during lip/palate development. PMID: 28767310
High NME1 expression is associated with well tumor differentiation in Digestive System Neoplasms PMID: 27518571
Hepatitis C Virus E1 protein expression and HCV infection induces pro-metastatic effect on cancer cells which is simultaneous to Nm23-H1 transcriptional down-regulation and Nm23-H1 protein degradation. PMID: 28376369
Meta-analysis showed that low expression of nm23-H1 is associated with poorer prognosis in patients with nasopharyngeal carcinoma, suggesting that it is a prognostic factor and potential biomarker for survival in nasopharyngeal carcinoma. PMID: 28614246
Nm23-H1 nuclear localized mainly in the G2/M phase and the nuclear Nm23-H1 promoted A549 cell proliferation in vitro. PMID: 28442010
Differential regulation of NM23-H1 may corroborate/abrogate EMT depending on the nature of stress, tumor microenvironment and cellular context. PMID: 28216015
large-scale and well-designed studies, which use uniform antibody and criterion of NM23 positive expression, are required to further validate the role of the NM23 in predicting gastric cancer progression. PMID: 28401162
Study reveals that NME1L may perform potent biological roles on the cellular behaviors through the extra N-terminal region as well as hexameric conformation. Because the N-terminal region itself has no effect on NF-kappaB signaling, the dimerization of NME1L is likely a pivotal process to confer the IKKbeta binding ability and subsequent regulation of NF-kappaB signaling on the region. PMID: 27094059
A strong association between NME1 heterozygous genotype and breast cancer risk in Kashmiri population PMID: 27509166
Down-regulation of Dyn1 activity enhances extracellular Nme1 in human colon tumor cell lines. PMID: 27449069
nm23-H1 and MMP-2 may be as an indicator for esophageal cancer metastasis and prognosis PMID: 27592483
Results confirmed that NME1L, but not NME1, is likely responsible for regulating breast cancer cell growth by inhibiting IGF1-stimulated ERK phosphorylation through N-terminal 25 amino acid-mediated interaction with KSR1. PMID: 26565392
CRC patients with NM23-positive tumors had a better prognosis, and thus NM23 expression maybe used as a key prognostic indicator for CRC. PMID: 26634527
NM23 expression is reduced in CRC tissues and low NM23 levels tightly correlate with higher Dukes stages, poorer differentiation grade, and positive lymph node metastases. PMID: 26825905
Fibronectin is an important effector of the motility-suppressing function of NME1 in melanoma cells. PMID: 25808322
Nm23 loss could be associated with a more favorable environment for the development and dissemination of breast cancer PMID: 25321081
Dual functions of NME1 in suppression of cell motility and enhancement of genomic stability in melanoma PMID: 25017017
Regulation of the metastasis suppressor Nm23-H1 by tumor viruses PMID: 25199839
nm23-H1 protein may be an important prognostic factor in peripheral T-cell lymphoma, not otherwise specified;the nm23-H1-positive group had significantly shorter overall survival (Review). PMID: 25501107
The case for extracellular Nm23-H1 as a driver of acute myeloid leukemia (AML) progression PMID: 25119778
Interaction between Nm23 and the tumor suppressor VHL PMID: 24915993
Using the described method for detecting histidine and aspartic acid phosphorylations and our prostate cancer progression cell system, the potential function of NM23-H1 in suppressing metastasis with a two-component regulation system is discussed PMID: 25373728
The metastasis suppressor NME1 regulates expression of genes linked to metastasis and patient outcome in melanoma and breast carcinoma. PMID: 25048347
NM23-H1 may participate in head and neck squamous cell carcinoma cell responses to cisplatin and be considered a potential therapeutic target. PMID: 25277180
NM23H1 gene suppresses hyperplasia and metastasis of prostate cancer, thereby improving the survival rate. PMID: 24858271
Overexpression of Nm23H1 did not affect tumorigenesis in nude mice assays, while overexpression of Nm23H2 enhanced tumor growth with elevated expression of the c-Myc proto-oncogene. PMID: 25748386
NDPK-A was not able to bind to model membranes mimicking the inner leaflet of plasma membrane, suggesting that its in vivo membrane association is mediated by a non-lipidic partner or other partners than the studied phospholipids. PMID: 25010650
Data shows that c-Abl and Arg induce NM23-H1 degradation by increasing expression and activation of cathepsin L and B, which directly cleave NM23-H1 in the lysosome. PMID: 24096484
NME1L is a potent antimetastatic protein and may be a useful weapon in the fight against cancers. PMID: 24811176
findings show NDPKs (NM23-H1/H2/H4) interact with and provide GTP to dynamins, allowing these motor proteins to work with high thermodynamic efficiency for membrane remodeling PMID: 24970086
abnormal lower expression of NME1 in endometriotic stromal cells leads to more secretion of IL-8 and VEGF, up-regulates the level of CD62E and CD105, and leads to angiogenesis of vascular endothelial cells, which promotes development of endometriosis. PMID: 24133580
Abnormally low expression of NME1 in endometrial stromal cells (ESCs) may be involved in the pathogenesis of endometriosis by up-regulating growth, adhesion and invasion of ESCs. PMID: 23856325
Positive expression of Nm23 protein was found in ovarian tissue in 77.7 % cases in BRCA1 mutation carriers and in 90.9 % in the control group. PMID: 23553196
NM23 suppressor protein may have a role in gastric carcinoma pathogenes PMID: 23725501
In laryngeal squamous cell carcinoma patients, the disease recurrence rate correlates inversely with nuclear nm23-H1 expression. PMID: 23768014
Data indicate that the activation of MAPK and PI3K pathways resulted in TGF-beta1 signaling by down-regulating Nm23-H1 expression and up-regulating the expression of TbetaRI and TbetaRII, favoring further activation of multiple signaling pathways. PMID: 23734265
High NM23-H1 expression is associated with radioresistance in nasopharyngeal carcinoma. PMID: 23464856
High NM23 expression is associated with sporadic colorectal cancer. PMID: 23679306
Reduced nm23 immunohistochemical expression is an independent negative prognostic factor for overall survival and progression-free survival in invasive breast cancer. PMID: 23818346
The crystal structure of oxidized Nm23-H1 is presented. It reveals the formation of an intramolecular disulfide bond between Cys4 and Cys145 that triggers a large conformational change that destabilizes the hexameric state. PMID: 23519676
NM23 demonstrated no discriminatory value in the interpretation of lymph node nevus rests. PMID: 23694823

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Subcellular Location

Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA.

Protein Families

NDK family

Tissue Specificity

Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to t

Database Links

HGNC: 7849

OMIM: 156490

KEGG: hsa:4830

STRING: 9606.ENSP00000013034

UniGene: Hs.463456

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Code	CSB-RA060362A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg), Hela whole cell lysate(30µg), MCF-7 whole cell lysate(30µg) All lanes: NDK A antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Exposure time：1min IHC image of CSB-RA060362A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing SH-SY5Y cells stained with CSB-RA060362A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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NME1 Recombinant Monoclonal Antibody

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Target Background