Product Details

Uniprot No.

Q9Y266

Target Names

NUDC

Alternative Names

Nuclear migration protein nudC (Nuclear distribution protein C homolog), NUDC

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human NUDC

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

6C3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

NUDC plays a fundamental role in nuclear migration and mitotic spindle organization, processes essential for proper cell division and neuronal development. Originally identified as a mammalian homolog of the Aspergillus nidulans nudC gene, this protein has become increasingly relevant to researchers investigating cytoskeletal dynamics, cell cycle regulation, and neurodevelopmental mechanisms.

This recombinant monoclonal antibody offers the reproducibility that demanding experimental workflows require. Because the antibody sequence is defined and produced through recombinant technology, you can expect consistent performance across different lots, eliminating the variability that can complicate long-term studies or multi-site collaborations. The rabbit IgG format, produced from clone 6C3 against a synthetic peptide derived from human NUDC, provides reliable target recognition with the specificity expected from monoclonal reagents.

Western blot validation demonstrates robust detection across a diverse panel of human cell lines including HeLa, 293, 293T, K562, and A549, giving you flexibility when selecting your experimental model. The antibody also shows cross-species reactivity with mouse and rat samples, confirmed through detection in brain tissue lysates from both species, which is particularly valuable for neuroscience applications requiring rodent models. The observed band at approximately 45 kDa runs slightly higher than the 39 kDa predicted molecular weight, a shift commonly attributed to post-translational modifications such as phosphorylation, which is known to regulate NUDC function during the cell cycle.

Whether you're exploring mitotic machinery, investigating neuronal migration defects, or characterizing cell cycle checkpoints, this antibody provides a dependable tool for your NUDC research across multiple experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Plays a role in neurogenesis and neuronal migration. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.

Gene References into Functions

High expression of NUDC is associated with prostate cancer. PMID: 27959429
the upregulation of miR-194 affects the hNUDC expression, leading to a downregulated expression of Mpl/ERK pathway proteins, and suppresses the mitosis and proliferation of NSCLC cells. PMID: 27035759
NudC is co-localized with Aurora B at the midbody and co-immunoprecipitated with Aurora B in mitosis. results suggest that that dynamic phosphorylation of NudC by Aurora B regulates cytokinesis. PMID: 27074040
NudC acetylation/deacetylation regulates mitotic progression and NudC deacetylation, likely through HDAC3, is critical for spindle function and chromosome congression. PMID: 24069238
Human nuclear distribution C is found to be closely associated with cell malignant hyperplasia in nasopharyngeal carcinoma. PMID: 21473139
NudC may be involved in the regulation of LIS1 stability by its chaperone function. PMID: 20675372
separate binding sites on the Mpl receptor for TPO and hNUDC identified PMID: 20529857
Mpl plays an important and specific role in mediating hNUDC-induced megakaryocyte proliferation and differentiation. PMID: 19560457
functions in mitosis and cytokinesis, in part by regulating microtubule organization at the midzone and midbody PMID: 12679384
PLK1 phosphorylation of NudC plays an essential role in cytokinesis. PMID: 12852857
Overexpression of NUDC inhibits prostatic neoplasm growth. PMID: 14676831
In this study, we report the binding of hNUDC to the extracellular domain of the thrombopoietin receptor (Mpl) as detected by the yeast two-hybrid system, GST pull-down, and co-immunoprecipitation. PMID: 16088917
NudC functions as both a substrate and a spatial regulator of Plk1 at the kinetochore to promote chromosome congression. PMID: 16860740
hNUDC induced significant changes in cellular morphology in NIH 3T3 cells stably transfected with pMpl-EGFP. Interestingly, these morphological changes were characteristic of cells undergoing megakaryocyte differentiation. PMID: 17658515
hNUDC induced a sustained activation of the extracellular signal-regulated protein kinases-1 and -2 (ERK1/2) as well as p38 mitogen-activated kinase (p38 MAPK) pathways PMID: 18288130

Hide All

Subcellular Location

Cytoplasm, cytoskeleton. Nucleus. Cytoplasm, cytoskeleton, spindle. Midbody.

Protein Families

NudC family

Tissue Specificity

Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart.

Database Links

HGNC: 8045

OMIM: 610325

KEGG: hsa:10726

STRING: 9606.ENSP00000319664

UniGene: Hs.263812

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
Please enter the characters shown. Click the image to refresh if unclear.

Code	CSB-RA906015A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, 293T whole cell lysate, A549 whole cell lysate, Rat Brain whole cell lysate, Mouse Brain whole cell lysate All lanes: NUDC antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 kDa Observed band size: 45 kDa
Have Questions?	Leave a Message or Start an on-line Chat

NUDC Recombinant Monoclonal Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background