Product Details

Uniprot No.

Q9BZD4

Target Names

NUF2

Alternative Names

Kinetochore protein Nuf2 (hNuf2) (hNuf2R) (hsNuf2) (Cell division cycle-associated protein 1), NUF2, CDCA1 NUF2R

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human NUF2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The development of the NUF2 recombinant monoclonal antibody involves a rigorous and standardized process to ensure its quality and specificity. B cells are initially isolated from an immunized animal, with the synthesized peptide derived from human NUF2 used as the immunogen. The extracted B cells are then subjected to total RNA extraction and cDNA synthesis through reverse transcription. The NUF2 antibody genes are amplified using specific primers targeting the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells to enable the production of the NUF2 recombinant monoclonal antibody. Following cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography, resulting in a highly purified preparation. Extensive characterization assays, including ELISA and WB analysis, are performed to validate the antibody's specificity and functionality, ensuring its accurate binding to human NUF2 protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.

Gene References into Functions

CDCA1 could be a prognostic marker in malignant melanoma patients. PMID: 27237743
Results provide evidence that CDCA1 plays a significant role in oral cavity carcinoma (OCC) tumorigenesis. Importantly, CDCA1 is associated with growth and survival of OCC cells where its elevated expression issignificantly associated with poor prognosis . PMID: 27499128
NUF2 modulates cell proliferation via cell cycle control in osteosarcoma Saos-2 cells. PMID: 27049259
found LncRNA AF339813 was positively regulated by NUF2 PMID: 26045769
NUF2 silencing by RANi (RNA interference) reduced the proliferation and colony formation ability of pancreatic cancer cells in vitro. PMID: 25370920
NUF2 plays a critical role in the regulation of HCC cell proliferation and apoptosis. PMID: 25374179
NUF2 was overexpressed in glioma tissues and differentially expressed in a series of glioma cell lines. Depletion of NUF2 by short-hairpin RNA inhibited cell growth in vitro and in vivo. PMID: 25481014
hnRNP K binds to the promoter region of NUF2 and activates its transcription. PMID: 25701787
These results suggest that CDCA1 could be a prognostic biomarker and a potential therapeutic target for colorectal cancers . PMID: 24247253
these findings demonstrated that three buried glutamic acid-lysine pairs, in concert with hydrophobic interactions of core residues, provide the major specificity and stability requirements for Hec1-Nuf2 dimerization PMID: 24129578
NDC80, NUF2 and PTN were significantly aberrantly overexpressed in serous adenocarcinomas. PMID: 23056589
These data suggest that the CH and tail domains of Hec1 generate essential contacts between kinetochores and microtubules in cells, whereas the Nuf2 CH domain does not. PMID: 21270439
cell growths of colorectal and gastric cancers after the siRNA-mediated knockdown of either CDCA1 or KNTC2 were significantly suppressed. PMID: 19878654
Data highlight a specific role for hNuf2 in kinetochore-microtubule attachment and suggest that hNuf2 is part of a molecular linker between the kinetochore attachment site and tubulin subunits within the lattice of attached plus ends. PMID: 12438418
NUF2 is a core component of the kinetochore outer plate essential for organizing microtubule attachment sites. PMID: 15548592
HsNUF2 and CENP-E are required for organization of stable microtubule-kinetochore attachment that is essential for faithful chromosome segregation in mitosis PMID: 17535814
CDCA1 may therefore be an ideal tumor-associated antigen useful for the diagnosis and immunotherapy of these cancer PMID: 18770861
alternative splicing variants, M-RIP, HYAL2, CDCA1, and MSMB genes showed differential expressions between cancer cells and corresponding normal tissues. PMID: 19081476
Chromosome congression in HSET + hNuf2 co-depleted cells required the plus-end directed motor CENP-E , which has been implicated in the gliding of mono-oriented kinetochores alongside adjacent K-fibres. PMID: 19525938

Hide All

Subcellular Location

Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization.

Protein Families

NUF2 family

Database Links

HGNC: 14621

OMIM: 611772

KEGG: hsa:83540

STRING: 9606.ENSP00000271452

UniGene: Hs.651950

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Code	CSB-RA248269A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate All lanes: NUF2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 55 kDa Observed band size: 55 kDa
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NUF2 Recombinant Monoclonal Antibody

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Target Background