Code | CSB-RA169000A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
IHC | 1:50-1:200 |
IF | 1:50-1:200 |
CUSABIO employed a meticulous process to produce the PAH recombinant monoclonal antibody. B cells were initially isolated from the spleen of an immunized animal, with the synthesized peptide derived from human PAH serving as the immunogen. RNA was extracted from the B cells and converted into cDNA through reverse transcription. Using the cDNA as a template, the gene encoding the PAH antibody was amplified with a degenerate primer and inserted into a vector. The vector was then introduced into host cells via transfection to facilitate the expression of the PAH recombinant monoclonal antibodies, which were subsequently harvested from the cell culture supernatant and purified using affinity chromatography. The PAH recombinant monoclonal antibody's specific reactivity with human PAH protein was verified through ELISA, IHC, and IF applications.
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