PDLIM1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA965164A0HU
Size	US$210
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Image	Western Blot , Positive WB detected in:PC-3 whole cell lysate(30µg), A431 whole cell lysate(30µg) All lanes: PDLIM1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time：120s IHC image of CSB-RA965164A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA965164A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

O00151

Target Names

PDLIM1

Alternative Names

C terminal LIM domain protein 1 antibody; C-terminal LIM domain protein 1 antibody; Carboxy terminal LIM domain protein 1 antibody; CLIM 1 antibody; CLIM1 antibody; CLP 36 antibody; CLP36 antibody; Elfin antibody; Enigma antibody; Enigma homolog antibody; hCLIM 1 antibody; hCLIM1 antibody; LIM domain protein CLP 36 antibody; LIM domain protein CLP-36 antibody; LIM domain protein CLP36 antibody; PDLI1_HUMAN antibody; PDLIM 1 antibody; Pdlim1 antibody; PDZ and LIM domain 1 antibody; PDZ and LIM domain protein 1 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human PDLIM1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

19E12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PDLIM1, also known as CLP-36 or Elfin, is a PDZ and LIM domain-containing protein that plays a critical role in cytoskeletal organization and cell signaling. This adapter protein localizes to actin stress fibers and focal adhesions, where it mediates protein-protein interactions essential for cellular architecture and mechanotransduction. PDLIM1 has garnered significant research interest for its involvement in epigenetics and nuclear signaling pathways, making it a valuable target for investigators studying cellular dynamics and signal transduction mechanisms.

This recombinant rabbit monoclonal antibody, clone 19E12, offers the consistency and reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human PDLIM1, the recombinant production method ensures sequence-defined specificity and eliminates the lot-to-lot variability often encountered with traditional hybridoma-derived antibodies. Affinity chromatography purification further enhances performance by removing non-specific immunoglobulins.

Validation studies demonstrate reliable detection across multiple experimental platforms. Western blot analysis confirms specific recognition of PDLIM1 at the expected 36 kDa molecular weight in both PC-3 prostate cancer and A431 epidermoid carcinoma cell lysates, with effective detection at 1:1000 dilution. Immunohistochemistry validation in paraffin-embedded human gastric cancer tissue shows clear staining at 1:100 dilution using standard citrate buffer antigen retrieval. Flow cytometry analysis in HeLa cells demonstrates distinct positive signal separation from isotype control, confirming utility for intracellular protein detection in fixed and permeabilized samples.

This versatile antibody supports researchers investigating cytoskeletal biology, focal adhesion dynamics, and nuclear signaling pathways, providing a dependable tool for characterizing PDLIM1 expression and localization across diverse experimental contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts.

Gene References into Functions

PDLIM1 was shown to interact with p75(NTR) in highly invasive patient-derived glioma stem cells/tumor-initiating cells and shRNA knockdown of PDLIM1 in vitro and in vivo results in complete ablation of p75(NTR)-mediated invasion. PMID: 26119933
downregulation of PDLIM1 in colorectal cancer samples correlated with reduced E-cadherin and membrane beta-catenin levels PMID: 26701804
Our results identify CLP36 as an important regulator of breast cancer cell migration and metastasis, and shed light on how increased CLP36 expression contributes to the progression of breast cancer. PMID: 24662836
Femtomolar Zn2+ affinity of LIM domain of PDLIM1 protein uncovers crucial contribution of protein-protein interactions to protein stability. PMID: 22922308
Robust co-regulation of tyrosine phosphorylation sites has been revealed on novel protein interactions between EGFR and PDLIM1. PMID: 22851037
PDLIM1 showed moderate parent-of origin effects for some single nucleotide polymorphisms in attention deficit disorder with hyperactivity. PMID: 21651830
Alpha-actinin-4 and CLP36 protein deficiencies contribute to podocyte defects in multiple human glomerulopathies PMID: 21680739
results establish the CLP-36 PDZ-LIM protein as an adapter, recruiting the Clik1 kinase to actin stress fibers in nonmuscle cells, and suggest that Clik1 represents a novel regulator of actin stress fibers PMID: 11973348
We present evidence for the exclusive protein expression of CLP36 in proliferative and early post-proliferative trophoblast cells. PMID: 16609848
Enigma protein shown to interact with alpha actinin 2 by yeast two-hybrid screening and immunochemistry (co-localization at myocardial Z-disks). PMID: 10861853
CLP-36 contains a PDZ- and LIM domain, is widely expressed, interacts with actinin-1, actinin-2 and actinin-4, and localizes to actin stress fibers. The gene (7 exons) is localised to contig NT_008878.2 on chromosome 10. PMID: 11110697

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line.

Tissue Specificity

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ov

Database Links

HGNC: 2067

OMIM: 605900

KEGG: hsa:9124

STRING: 9606.ENSP00000360305

UniGene: Hs.368525

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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