PDLIM1 Antibody

Datasheet

Code	CSB-PA017731ESR2HU
Size	US$166
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Image	Western blot All lanes: PDZ and LIM domain protein 1 antibody at 10μg/ml + Mouse lung tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 36 kDa Observed band size: 36 kDa Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA017731ESR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA017731ESR2HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human pancreatic tissue using CSB-PA017731ESR2HU at dilution of 1:100 Immunoprecipitating PDLIM1 in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA017731ESR2HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000) Lane 2: CSB-PA017731ESR2HU (4μg) + HepG2 whole cell lysate (500μg) Lane 3: HepG2 whole cell lysate (20μg)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PDLIM1 Polyclonal antibody

Uniprot No.

O00151

Target Names

PDLIM1

Alternative Names

C terminal LIM domain protein 1 antibody; C-terminal LIM domain protein 1 antibody; Carboxy terminal LIM domain protein 1 antibody; CLIM 1 antibody; CLIM1 antibody; CLP 36 antibody; CLP36 antibody; Elfin antibody; Enigma antibody; Enigma homolog antibody; hCLIM 1 antibody; hCLIM1 antibody; LIM domain protein CLP 36 antibody; LIM domain protein CLP-36 antibody; LIM domain protein CLP36 antibody; PDLI1_HUMAN antibody; PDLIM 1 antibody; Pdlim1 antibody; PDZ and LIM domain 1 antibody; PDZ and LIM domain protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human PDZ and LIM domain protein 1 protein (1-329AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IP	1:200-1:2000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts.

Gene References into Functions

PDLIM1 was shown to interact with p75(NTR) in highly invasive patient-derived glioma stem cells/tumor-initiating cells and shRNA knockdown of PDLIM1 in vitro and in vivo results in complete ablation of p75(NTR)-mediated invasion. PMID: 26119933
downregulation of PDLIM1 in colorectal cancer samples correlated with reduced E-cadherin and membrane beta-catenin levels PMID: 26701804
Our results identify CLP36 as an important regulator of breast cancer cell migration and metastasis, and shed light on how increased CLP36 expression contributes to the progression of breast cancer. PMID: 24662836
Femtomolar Zn2+ affinity of LIM domain of PDLIM1 protein uncovers crucial contribution of protein-protein interactions to protein stability. PMID: 22922308
Robust co-regulation of tyrosine phosphorylation sites has been revealed on novel protein interactions between EGFR and PDLIM1. PMID: 22851037
PDLIM1 showed moderate parent-of origin effects for some single nucleotide polymorphisms in attention deficit disorder with hyperactivity. PMID: 21651830
Alpha-actinin-4 and CLP36 protein deficiencies contribute to podocyte defects in multiple human glomerulopathies PMID: 21680739
results establish the CLP-36 PDZ-LIM protein as an adapter, recruiting the Clik1 kinase to actin stress fibers in nonmuscle cells, and suggest that Clik1 represents a novel regulator of actin stress fibers PMID: 11973348
We present evidence for the exclusive protein expression of CLP36 in proliferative and early post-proliferative trophoblast cells. PMID: 16609848
Enigma protein shown to interact with alpha actinin 2 by yeast two-hybrid screening and immunochemistry (co-localization at myocardial Z-disks). PMID: 10861853
CLP-36 contains a PDZ- and LIM domain, is widely expressed, interacts with actinin-1, actinin-2 and actinin-4, and localizes to actin stress fibers. The gene (7 exons) is localised to contig NT_008878.2 on chromosome 10. PMID: 11110697

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line.

Tissue Specificity

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ov

Database Links

HGNC: 2067

OMIM: 605900

KEGG: hsa:9124

STRING: 9606.ENSP00000360305

UniGene: Hs.368525

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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